| 胆木总生物碱的含量测定方法研究 |
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| 引用本文： | 杨卫丽,赖伟勇,高靖淋,刘侠. 胆木总生物碱的含量测定方法研究[J]. 中国当代医药, 2012, 19(18): 67-68 |
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| 作者姓名： | 杨卫丽 赖伟勇 高靖淋 刘侠 |
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| 作者单位： | 海南医学院,海南省热带药用植物研究开发重点实验室,海南海口,571101 |
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| 基金项目： | 海南省海口市重点科技计划项目 |
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| 摘 要： | 目的建立胆木总生物碱含量测定方法。方法采用紫外分光光度法测定胆木中总生物碱的含量。结果建立了胆木总生物碱的含量测定方法,测定波长为222nm,标准曲线为Y=62．841X＋0．0363,R=0．9997（n=9）,线性范围0．00096-0．0192mg／mL,平均回收率为100．45％,RSD为0．62％（n=9）。结论该方法稳定、可靠、专属性强,可用于胆木总生物碱的含量测定,为胆木的质量控制提供了新方法。
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| 关 键 词： | 胆木 总生物碱 含量 测定 |
Study on the determination method of total alkaloids in Nauclea officinalis |
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| YANG Weili , LAI Weiyong , GAO Jinglin , LIU Xia. Study on the determination method of total alkaloids in Nauclea officinalis[J]. http://www.botanicus.org/, 2012, 19(18): 67-68 |
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| Authors: | YANG Weili LAI Weiyong GAO Jinglin LIU Xia |
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| Affiliation: | Hainan Medical College(Key Laboratory of Hainan Institute for Research and Development of Topical Plant Medical),Haikou 571101,China |
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| Abstract: | Objective To establish the content determination method of total alkaloids in Nauclea officinalis.Methods Used UV spectrophotometry to detect total alkaloids of Nauclea officinalis.Results The method of determinating the content of total alkaloids in Nauclea officinalis was established.Wavelength:222 nm,Standard curve:Y = 62.841X + 0.036 3,R = 0.999 7(n = 9) in the range of 0.000 96～0.019 2 mg/mL,the average recovery was 100.45%,and the RSD was 0.62%(n = 9).Conclusion This method is simple,reliable,precise and provide a scientific basis for the quality controlling of total alkaloids in Nauclea officinalis. |
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| Keywords: | Nauclea officinalis Total alkaloids Content Determination |
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