|
|||||
|
|
| LC-MS/MS 测定大鼠血浆中香柑内酯及其药代动力学研究 | |
| 引用本文： | 马玉凤,李文,胡芳弟,石义凯,毕映燕,封士兰.LC-MS/MS 测定大鼠血浆中香柑内酯及其药代动力学研究[J].中国中药杂志,2012,37(3):381-388. |
| 作者姓名： | 马玉凤 李文 胡芳弟 石义凯 毕映燕 封士兰 |
| 作者单位： | 1. 兰州大学药学院,甘肃兰州 730000;陕西省汉中市食品药品检验所,陕西汉中 723000 2. 兰州大学药学院,甘肃兰州,730000 3. 兰州大学药学院,甘肃兰州 730000;兰州大学甘肃省新药临床前研究重点实验室,甘肃兰州 730000 |
| 基金项目： | 甘肃省科技计划项目(1011WCGA162﹚;兰州大学中央高校基本科研业务费专项资金项目(lzujbky-2010-191);教育部"春晖计划"合作科研项目(Z2009-1-62002);兰州大学国家大学生创新性实验计划项目(101073039) |
| 摘 要： | 目的:建立测定香柑内酯(berg)血浆药物浓度的液相色谱-电喷雾离子化-质谱(LC-ESI-MS)联用的分析方法,并探讨其在大鼠体内的药动学。方法:采用Hypersil ODS柱(4.6 mm×250 mm,5 mm),流动相甲醇-水(77.5∶2.5),流速0.8mL.min-1,柱温30℃,检测波长300 nm。结果:Berg在8.12～162.4μg.L-1(r=0.999 9)线性关系良好。方法学考察均符合要求。日内、日间变异系数(RSD)均<10%,精密度和准确度等均符合生物样品分析的要求。结论:该法准确、灵敏、特异,适用于berg的体内定量分析,berg在大鼠体内药动学过程属于一级吸收二室模型。 |
| 关 键 词： | LC-MS/MS 香柑内酯 药代动力学 |
| 收稿时间： | 2011/11/29 0:00:00 |
Phamacokinetic study of bergapten in rats plasma by LC-MS/MS |
|
| MA Yufeng,LI Wen,HU Fangdi,SHI Yikai,BI Yingyan and FENG Shilan.Phamacokinetic study of bergapten in rats plasma by LC-MS/MS[J].China Journal of Chinese Materia Medica,2012,37(3):381-388. | |
| Authors: | MA Yufeng LI Wen HU Fangdi SHI Yikai BI Yingyan and FENG Shilan |
| Institution: | School of Pharmacy, Lanzhou University, Lanzhou 730000, China;Hanzhong Institute of Food and Drug Control, Hanzhong 723000, China;School of Pharmacy, Lanzhou University, Lanzhou 730000, China;School of Pharmacy, Lanzhou University, Lanzhou 730000, China;School of Pharmacy, Lanzhou University, Lanzhou 730000, China;School of Pharmacy, Lanzhou University, Lanzhou 730000, China;School of Pharmacy, Lanzhou University, Lanzhou 730000, China;Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou 730000, China |
| Abstract: | Objective: To determine bergapten’s concentration in plasma and observe its pharmacokinetics in rats using a combined LC-MS/MS analytical method.Method: Blood samples were separated on a Hypersil ODS column(4.6 mm×250 mm,5 mm) at a temperature of 30 ℃,and mobile phase consisted of water and methanol(22.5∶77.5) at a flow rate of 0.8 mL·min-1.Result: The methodological study showed a good linear relationship of 8.12-162.4 g ·L-1(r=0.999 9).The inner and inter-days relative standard deviations were both less than 10%,indicating legitimate precise and accuracy to the requirement of biological sample analysis.Conclusion: The method is suitable for in vivo quantitative analysis for bergapten due to its accuracy,sensitivity and specificity.The pharmacokinetic process in rats forms a two-compartment model with first-order absorption. |
| Keywords: | LC-MS/MS bergapten phamacokinetics |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |