|
|||||
|
|
| 吴茱萸遗传多样性的 AFLP 分析 | |
| 引用本文： | 魏宝阳,曹亮,李顺祥,黄丹,卢向阳,虢小翊,周晋.吴茱萸遗传多样性的 AFLP 分析[J].中国中药杂志,2012,37(3):278-282. |
| 作者姓名： | 魏宝阳 曹亮 李顺祥 黄丹 卢向阳 虢小翊 周晋 |
| 作者单位： | 1. 湖南中医药大学药学院,湖南长沙 410208;湖南农业大学生物科学技术学院,湖南长沙 410128 2. 湖南中医药大学药学院,湖南长沙 410208;湖南省农业科学院农业生物资源利用研究所,湖南长沙 410125 3. 湖南中医药大学药学院,湖南长沙 410208;湖南中医药研究院中药研究所,湖南长沙 410013 4. 湖南农业大学生物科学技术学院,湖南长沙,410128 5. 4.湖南省农业科学院农业生物资源利用研究所,湖南长沙,410125 6. 湖南中医药大学药学院,湖南长沙,410208 |
| 基金项目： | 国家自然科学基金项目(30973877);国家"十一五"科技支撑计划项目(2006BAI09B02);湖南省科学技术厅科技项目(2008SK3087);国家中医药管理局"中药植物学"重点学科项目(国中医药发[2009]30号);湖南省高校"中药新药创制与资源综合利用"科技创新团队项目(湘教通[2010]212号);湖南省"中药学"重点学科项目 |
| 摘 要： | 目的:应用AFLP技术对吴茱萸的遗传背景进行研究,分析不同产区吴茱萸的遗传特性差异。方法:试剂盒提取吴茱萸幼嫩叶片基因组DNA,构建AFLP实验体系并筛选引物,对来自全国5个省份的46份样品进行遗传背景的研究;用NT-SYS-pc 2.1软件对AFLP扩增结果进行聚类分析。结果:从72对AFLP引物中优选6对用于吴茱萸遗传背景的研究,通过研究发现在相关系数为0.53时,浙江地区的吴茱萸石虎变种样品与其他地区样品分开;石虎变种遗传差异比吴茱萸更显著并呈现出较强的地缘相关性。结论:吴茱萸遗传背景差异明显,AFLP分析方法可有效鉴别不同品种的吴茱萸,并能检测到地区间的差异。 |
| 关 键 词： | 吴茱萸 AFLP 道地药材 聚丙烯酰胺凝胶电泳 银染 |
| 收稿时间： | 2011/8/14 0:00:00 |
AFLP analysis of genetic diversity of Evodia rutaecarpa |
|
| WEI Baoyang,CAO Liang,LI Shunxiang,HUANG Dan,LU Xiangyang,GUO Xiaoyi and ZHOU Jin.AFLP analysis of genetic diversity of Evodia rutaecarpa[J].China Journal of Chinese Materia Medica,2012,37(3):278-282. | |
| Authors: | WEI Baoyang CAO Liang LI Shunxiang HUANG Dan LU Xiangyang GUO Xiaoyi and ZHOU Jin |
| Institution: | College of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China;College of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;College of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China;College of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China;Hunan Academy of Traditional Chinese Medicine, Changsha 410013, China;College of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China;Hunan Academy of Traditional Chinese Medicine, Changsha 410013, China;College of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;College of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China;College of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China |
| Abstract: | Objective: To study the genetic background of Evodia rutaecarpa by AFLP, and analyze the genetic diversity of E. rutaecarpa from different areas. Method :E. rutaecarpa genomic DNA was extracted. The AFLP reaction system was established and AFLP primer pairs were chosen for the analysis. Forty-six individuals of E. rutaecarpa which from five provinces were analyzed by AFLP. The NTSYS-pc 2.1 software was used for cluster analysis. Result: Six out of the original 72 pairs of primers were optimized for the study; AFLP analysis revealed the similarity coefficient of 0.53, the samples of E. rutaecarpa var. officinalis from Zhejiang province was separated from other accessions; E. rutaecarpa var. officinalis also showed more pronounced genetic variation than the E. rutaecarpa, and strong geo-related relevance. Conclusion: Variance of genetic background of E. rutaecarpa are large, AFLP analysis method can obviously identify different varieties of E. rutaecarpa, and can detect the genetic characteristics of inter-regional differences. |
| Keywords: | Evodia rutaecarpa AFLP geo-authentic crude drugs polyacrylamide gel electrophoresis silver staining |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |