来氟米特对实验性变态反应性脑脊髓炎小鼠T细胞亚群及Th1型细胞因子的影响

目的探讨来氟米特（Leflunomide，LEF）对实验性变态反应性脑脊髓炎（EAE）小鼠模型发病防治作用及可能的免疫学机制。方法将30只雌性C57BL／6小鼠随机分为EAE对照组及高、低剂量LEF防治组，每组10只。通过注射髓鞘少突胶质细胞糖蛋白35—55（MOG35—55）免疫介导构建EAE模型。从造模前三天开始起EAE对照组及高、低剂量LEF防治组分别予以生理盐水和LEF8mg／kg／d与2mg／kg／d灌胃，连续10天。观察小鼠发病情况及外周血中T细胞亚群及IFN-扎IL-2水平的变化。结果两组使用LEF小鼠临床症状较轻，发病高峰期外周血T细胞亚群CD4＋、CD8＋分布比例较EAE对照组明显升高（P〈O．01），CD4＋／CD8＋值明显降低（P〈O．01）；发病高峰期外周血IL-2、INF-7含量较EAE对照组明显降低（P〈O．01），且高剂量组降低更明显（P〈O．01）。结论LEF对EAE具有防治作用，且效果与LEF剂量有关。其防治作用可能与调节T淋巴细胞亚群分布及抑制IFN—y、IL-2水平有关。

Effect of Leflunomide on T cell subsets and Thl-type cytokines in experimental allergic encephalomyelitis mice

Objective To investigate the effect of leflunomide on experimental allergic encephalomyelitis mice and the possible immunological mechanisms. Methods 30 female C57BL/6 mice were randomly divided into control group, the high and low close leflunomide treatment group, n= 10. By injection of myelin oligodendrocyte glyeoprotein 35-55 im- mune-mediated build EAE model. From the beginning three days before modeling EAE, control group and the high and low dose treatment group were feed saline, leflunomide 8 mg/kg/d, leflunomide 2mg/kg/d for 10 days. Incidence was observed in mice and peripheral blood T cell subsets and IFN-,IL-2 levels were detected. Results Each treatment group had mild clinical symptoms, and peripheral blood T cell subsets CD4＋, CDS＋ distribution were higher than EAE con- trol group （P〈0.01）, CD4＋/CDS＋ value was lower （P〈O, 01） ; peripheral blood IL- 2, INF-y levels were significant- ly lower than EAE control group （P〈0. 01）, and high dose reduction is more significant （P〈0.01）. Conclusion Le- flunomide has preventive effect on EAE , and the effect is dose related with the leflunomide. Its preventive effect may be related to the regulation of T lymphocyte subsets and inhibition of IFN- and IL-2.