宫颈扩张过程中单核细胞趋化蛋白-1表达的意义

目的:观察单核细胞趋化蛋白-1(MCP-1)在分娩期宫颈扩张不同程度时母血、子宫肌层组织和胎盘组织中的表达,探讨MCP-1在宫颈扩张过程中的作用。方法:选择临产后急诊行剖宫产终止妊娠的初产妇42例,根据术前30min宫颈扩张程度分为A组10例(宫颈口开大<2cm),B组10例(宫颈口开大2~4cm),C组10例(宫颈口开大4~6cm),D组12例(宫颈口开大>6cm)。酶联免疫吸附法测定产妇血清中MCP-1水平;用半定量RT-PCR法测定子宫下段肌层及胎盘组织中MCP-1mRNA的表达。结果:C组和D组血清MCP-1水平和组织中MCP-1mRNA含量显著高于A组和B组(P<0.01),B组组织中MCP-1mRNA含量显著高于A组(P<0.05);4组产妇MCP-1的表达量与宫颈扩张程度呈正相关。结论:MCP-1在宫颈扩张过程中表达增加,在宫颈扩张过程中起重要作用。

Expression and effect of monocyte chemotactic protein-1 during delivery

Objective:To determine the expression of monocyte chemotactic protein-1(MCP-1)in the blood,lower uterine segment and placenta during parturition at term and to investigate its correlation with the stage of cervical dilatation.Methods:Biopsy specimens were taken from the lower uterine segment of 42 women undergoing cesarean section at term.The stage of cervical dilatation 30 minutes before the time of cesarean section was < 2cm ( group A,n=10),2 to< 4 cm ( group B,n=10),4 to 6 cm ( group C,n=10) or > 6cm ( group D,n= 12).Enzyme linked immunosorbent assay ( ELISA ) was used to measure concentrations of MCP-1 in the blood.The expression of MCP-1 mRNA in lower segment of uterus and placenta from the group of cesarean section were studied by semi-quantitative RT-PCR.Results:The concentration of MCP-1 in serum and the expression of MCP-1 mRNA in tissue were significantly greater at group C and group D than group A and group B (P< 0.01).The group B was also higher than group A in tissue(P<0.05),the expression of MCP-1 was correlated with cervical dilatation.Conclusion:Cervical dilatation is associated with up-regulation of MCP-1 in the lower uterine segment.MCP-1 is a key mediator in maturation and dilatation of uterine cervix.