Expression of the cell surface antigen detected by the monoclonal antibody A7 in pancreatic carcinoma cell lines |
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| Authors: | Eigo Otsuji Toshiharu Yamaguchi Nozomi Yamaguchi Kunihiko Koyama Jiro Imanishi Nobuki Yamaoka Toshio Takahashi |
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| Affiliation: | (1) The First Department of Surgery, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji Agaru Kajiicho 465, Kamigyo-ku, 602 Kyoto, Japan;(2) the Department of Microbiology, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji Agaru Kajiicho 465, Kamiygo-ku, 602 Kyoto, Japan;(3) the Department of Preventive Medicine, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji Agaru Kajiicho 465, Kamigyo-ku, 602 Kyoto, Japan |
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| Abstract: | In a previous study, we used a murine monoclonal antibody, A7, against human colon carcinoma as a drug-carrier to treat colorectal cancer.1 In the present study, we found that MAb A7 also reacted immunohistochemically with 73% of human pancreatic carcinoma cell lines, with the A7 antigen mainly being detected on the cell surface. However, the A7 antigen was found in only 9% of the spent media of these human pancreatic carcinoma cell lines by ELISA. On the other hand, the positive incidence of CA19-9, POA, ferritin, CEA, DU-PAN-2 and SLX in those spent media was 100%, 64%, 64%, 55%, 55% and 36%, respectively. These results suggest that the A7 antigen may only rarely be shed into the sera of pancreatic cancer patients, in which case MAb A7 could be a suitable drug-carrier in targeting chemotherapy for pancreatic cancer patients. |
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| Keywords: | monoclonal antibody A7 pancreatic tumor marker pancreatic carcinoma cell line immunotargeting chemotherapy |
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