益肾胶囊对糖尿病肾病大鼠肾组织SOCS3、TGF-β_1、MCP-1表达的影响

目的:观察益肾胶囊对糖尿病肾病(DN)大鼠肾组织细胞因子信号抑制因子-3(SOCS3)、TGF-β1、MCP-1表达水平的影响。方法:40只健康雄性Wistar大鼠随机分为正常对照组、DN模型组(模型组)、益肾胶囊组、氯沙坦组,每组各10只。单侧肾切除加链脲佐菌素(STZ)腹腔注射建立DN大鼠模型,益肾胶囊组每只灌胃益肾胶囊(625mg·kg-1.d-1),氯沙坦组每只灌胃氯沙坦钾片(30mg·kg-1.d-1),正常对照组及模型组每日给予等量蒸馏水。测定各组大鼠体重、24h尿蛋白定量、尿微量白蛋白、血肌酐(Scr)、尿素氮(BUN),并计算内生肌酐清除率(Ccr);取肾组织行病理组织学观察,免疫荧光法检测肾组织中SOCS3、TGF-β1、MCP-1表达水平。结果:实验12周末,模型组大鼠24h尿蛋白定量、尿微量白蛋白、Scr、BUN均高于正常对照组(P<0.05),肾组织中SOCS3、TGF-β1、MCP-1表达水平高于正常对照组(P<0.05);治疗组大鼠24h尿蛋白定量、尿微量白蛋白、Scr、BUN均低于模型组(P<0.05),Ccr较模型组升高(P<0.05),而低于正常对照组(P<0.05),肾组织中SOCS3表达水平明显高于模型组(P<0.05),而TGF-β1、MCP-1的表达受到抑制(P<0.05);两治疗组间比较差异无统计学意义。结论:益肾胶囊可能通过上调SOCS3在肾组织的表达,抑制了TGF-β1、MCP-1的表达,从而减轻了DN大鼠肾小球硬化和间质纤维化的程度,延缓DN的进展。

Effect of Yishen Capsule on Expression of SOCS3 in Renal Tissue of Rats with Diabetic Nephropathy

Objective：To investigate the effect of YiShen capsule on the expression of SOCS3,TGF-β1 and MCP-1 in renal tissue of rats with diabetic nephropathy.Methods：40 Wistar rats were divided randomly into four groups：control,model,Yishen capsule treatment and losartan treatment,10 rats each group.The rat models were induced by right nephrectomy and peritoneal injection of low-dosage streptozotocin,the rats were treated with yishen capsule（625 mg·kg^-1·d^-1）/Losartan potassium（30 mg·kg^-1·d^-1）by a stomach tube in treatment group,and same amount of water was fed in control and model group.Body weight,urinary protein/24 h,microalbuminuria,serum creatinine（Scr）,blood urea nitrogen（BUN）,and endogenous creatinine clearance rate（Ccr）were measured,and optics microscope was adopted to observe the renal pathological changes.Expression levels of SOCS3,TGF-β1 and MCP-1 in renal tissue of rats were detected by immunofluorescence microscope.Results：In 12w,urinary protein/24 h,microal-buminuria,Scr,BUN in model group were higher significantly compared with contol group（respectively,P〈0.05）.The expression of SOCS3,TGF-β1 and MCP-1 of renal tissue in model group were higher significantly compared with control group（respectively,P〈0.05）.After treatment of Yishen capsule /Losartan potassium,urinary protein/24 h,microalbuminuria,Scr,BUN were reduced（respectively,P〈0.05）.Ccr were higher compared with model group（respectively,P〈0.05）,but lower than control group（respectively,P〈0.05）.The expression of SOCS3 of renal tissue in treatment group were higher significantly（respectively,P〈0.05）.The expression of TGF-β1 and MCP-1 of renal tissue in treatment group were lower（respectively,P〈0.05）.Two treatment groups weot statistically significant.Conclusion：After treatment of Yishen capsule /Losartan potassium,the expression of SOCS3 of renal tissue were higher significantly,the expression of TGF-β1 and MCP-1 were decreased.To reduced glomerular sclerosis and interstitial fibrosis,delay the progress of the diabetic nephropathy effectively.