血管性痴呆小鼠海马组织学改变及乙酰胆碱酯酶活性变化特征

目的:探讨血管性痴呆(VD)小鼠海马组织病理学改变及乙酰胆碱酯酶病理(AchE)活性的变化特征.方法:将50只小鼠随机分为假手术组和模型组。模型组采用双侧颈总动脉反复缺血再灌注法制备VD模型,术后第29天和第30天测试学习记忆成绩,术后第30天检测海马CA1区组织学变化及AchE含量。结果:VD模型组小鼠学习记忆成绩较假手术组明显下降;海马CA1区锥体细胞数目减少,细胞核体积变小;且模型组小鼠海马AchE活性明显低于假手术组(P<0.01)。结论:小鼠海马组织内的AchE活性下降参与了VD的形成,进一步导致VD小鼠的学习和记忆障碍。

Study on the pathological changes and the activities of acetylcholinesterase in hippocampus of mice with vascular dementia

Objective：To study the changes of the pathology and the activity of acetylcholinesterase（AchE） in hippocampus of mice with vascular dementia（VD）.Methods：50 kunming mice were randomly divided into model group and sham-operated group.In model group,the VD model were established by three repeated times ischemia-reperfusion through the ligation of the bilateral common carotid arteries.The abilities of learning and memory of mice were investigated by step-down test and water maze test in all animals.The changes of the pathology and the activity of AchE in CA1 area of hippocampus were measured on the 30th day after the operation in 2 groups of mice.Results：Compared with the shame group,the grades of learning and memory of mice in model group were lower;the number of pyramidal cells in CA1 area of hippocampus decreased and their nucleus became smaller ;the activity of AchE in CA1 area of hippocampus in model group decreased evidently（P〈0.01）.Conclusion：The decreased activities of AchE in hippocampus participates the cognitive damage in VD and leads to the dysfunction of learning and memory of mice in VD.