脂质、血红蛋白、胆红素标本对乙肝病毒DNA荧光定量测定的影响

目的:探讨脂血、高胆红素和溶血标本对乙肝病毒DNA(HBVDNA)荧光定量测定结果的影响。方法:将乙肝大三阳高脂血和非脂血、溶血血清和未溶血血清同时作HBVDNA荧光定量检测;将HBVDNA阴性黄疸血清和HBVDNA阴性正常血清与来自同一份乙肝大三阳血清混合,在相同条件下进行HBVDNA荧光定量。结果:乙肝大三阳溶血与未溶血样本HBVDNA含量都在同一数量级。乙肝大三阳高脂血的HBVDNA含量明显低于对照标本。高黄疸血清、正常对照血清与相同的HBVDNA阳性模板组合后所测得的HBVDNA结果无差异。结论:脂血对HBVD-NA定量测定有严重干扰;溶血样本、高胆红素样本对HBVDNA测定结果无影响。

Investigating on Effect of Hemolytic Blood, Serum Lipids and Serum Bilirubin on Real-Time Flourimetry PCR of HBVDNA

Objective: To investigate the effect of the high lipid, high bilirubin and hemolytic blood specimens on real time fluorimetry PCR of HBVDNA.Method: The serums from the case that were HBsAg+,HBeAg+ and anti-HBc+, and the serums from the same case but those were high lipids or hemolytic blood were tested by real-time fluorimetry PCR of HBVDNA parallel;Collecting serums from the case that HBVDNA was negative but with high bilirubin and serum that HBVDNA was negative but with nomorl level of bilirubin,mixed them with a same positive serum of HBVDNA respectively, and then tested by real time flurimetry PCR of HBVDNA parallel.Results: 12 sample of the hemolytic blood and 9 high serum bilirubin have no siginificant change compared with the contrast in HBVDNA; 9 sample of the high lipids were lower than the contrast obviously.Conclusion: The hemolytic blood and the serum with high bilirubin have no affecting to the real time fluorimetry PCR of HBVDNA.However,the sample of high lipids will affect on it seriously.