人恶性肿瘤培养细胞抗癌基因P53功能状态与肿瘤化疗敏感性的关系

目的：探讨人恶性肿瘤培养细胞抗癌基因P53功能状态与化疗敏感性的关系。方法：采用用大肠癌、肺癌、食管癌、多发性骨髓瘤、白血病5种人体肿瘤培养细胞共20株，经P53功能检测方法确认P53功能状态，用MTT法检测P53不同功能状态下,各种肿瘤细胞对化疗药VP－16的敏感性及差异。结果：以VP－16的IC50值计算，P53功能正常的肿瘤细胞株大多比同类肿瘤P53功能丧失的细胞株高58倍，显示出较高的化疗敏感性。结论：人体多种恶性肿瘤培养细胞P53功能状态直接影响其化疗敏感性。

Relationship between the P53 functional status of human tumor cell lines and their chemosensitivities to etoposide

Objective:To study the relationship between the functional status of the tumor suppressor gene P53 of human tumor cell lines and their chemosensitivities.Methods:20 human tumor cell lines from 5 different tumor types including colorectal carcinoma,lung carcinoma, esophageal carcinoma, multiple myeloma and leukemia were used in this study.The P53 status was determined by using functional analysis of separated alleles in yeast(FASAY).By MTT(methyl thiazolyl tetrazolium) method,the chemosensitivity to etoposide was tested in all the cell lines and in different P53 functional status.Results:The tumor cell lines in which P53 function is normal showed higher chemosensitivity than those cell lines from the same tumor type in which P53 function is lost.IC 50 of the former is 58 times lower than that of the latter.Conclution:The P53 functional status of human tumor cell lines from many tumor types directly influences their chemosensitivities.