含氯消毒剂对多重耐药鲍曼不动杆菌杀灭效果的研究

目的 探讨不同浓度的含氯消毒剂对多重耐药鲍曼不动杆菌的杀灭效果.方法 收集2008年11月-2009年12月住院患者样本中分离的多重耐药鲍曼不动杆菌共30株,采用悬液定量杀灭试验方法研究含氯消毒剂对这些菌株的杀灭效果.多组间数据比较采用单因素方差分析.结果 含有效氯400 mg/l和600 mg/l的消毒剂作用10 min,含有效氯800 mg/l的消毒剂作用3 min以及含有效氯1000 mg/l的消毒剂作用1 min,对30株多重耐药鲍曼不动杆菌临床分离株的杀灭对数值(kl)均≥5.00,杀灭有效率均能达到100％；不同有效氯浓度的消毒液作用相同时间对多重耐药鲍曼不动杆菌的杀灭对数值,除作用时间10 min组外,差异均具有统计学意义(f=72.72、64.79和32.33,p=0.00)；相同有效氯浓度的消毒液作用不同时间对多重耐药鲍曼不动杆菌的杀灭对数值,除1000 mg/l组外,差异均有统计学意义(f=110.42、20.41和3.20,p=0.00、0.00和0.03).结论 目前临床常用的消毒剂含有效氯500 ～ 1000 mg/l,作用10 ～ 30 min,对多重耐药鲍曼不动杆菌可达到理想的杀灭效果.abstract：objective to evaluate the killing efficacy of chlorine-releasing agents (cras) with different concentrations on multidrug-resistant acinetobacter baumannii.methods totally 30 clinical strains of multidrug-resistant acinetobacter baumannii were collected from november 2008 to december 2009.killing efficacy of cras on these strains was evaluated by quantitative suspension test.the one-way analysis of variance was performed.results the log values of killing to 30 strains of multidrug-resistant acinetobacter baumannii were all ≥5.00,when the bacteria were exposed to available chlorine concentrations 400 mg/l of cras for 10 min,600 mg/l for 10 min,800 mg/l for 3 min and 1000 mg/l for 1 min,respectively.and effective rates were all 100％. furthermore,there were significant differences among different available chlorine concentrations exposed for the same time ( except 10 min) ( f =72.72,64.79 and 32.33,p =0.00),and among different exposure time in the same available chlorine concentration ( except 1000 mg/l) ( f =110.42,20.41 and 3.20,p=0.00,0.00 and 0.03).conclusion satisfactory killing efficacy of cras to multidrug-resistant acinetobacter baumannii can be achieved with available chlorine concentration 500 mg/l to 1000 mg/l and exposure time 10 min to 30 min.

killing efficacy of chlorine-releasing agents on multidrug-resistant acinetobacter baumannii