乳腺癌ER、PR、c-erbB-2和nm23表达的相关性及临床病理意义

目的研究乳腺癌癌基因c-erbB-2、抑癌基因nm23和雌激素(ER)、孕激素受体(PR)的表达及意义。方法应用免疫组化SP法检测126例浸润性乳腺癌c-erbB-2、nm23及雌、孕激素受体的表达。结果①ER的表达与癌组织分化、肿瘤TNM分期及肿瘤大小呈负相关,小叶癌ER阳性率高于导管癌,而与患者年龄及淋巴结转移无显著相关性。②PR的表达与癌组织分化、肿瘤TNM分期呈负相关,与肿瘤大小、肿瘤类型、患者年龄及淋巴结转移无显著相关性。③c-erbB-2表达与肿瘤TNM分期、组织学分级、肿瘤大小及淋巴结转移呈正相关,与患者年龄、肿瘤类型无显著相关性。④乳腺癌Ⅰ期nm23表达的阳性率及阳性强度明显高于Ⅱ、Ⅲ期癌,nm23表达与组织学分级、淋巴结转移呈负相关,而与肿瘤大小及类型无显著相关性。⑤ER与PR表达呈正相关;nm23表达与ER、PR表达呈正相关,与c-erbB-2表达呈负相关;癌组织c-erbB-2与ER表达呈负相关,与PR表达无显著相关性。结论ER、PR、c-erbB-2及nm23的联合检测能够较好地反映乳腺癌病理生物学特征。

Expression of c-erbB-2,nm23,estrogen receptor and progestogen receptor in breast carcinoma:their correlation and dinicopathological significance

Objective To investigate the expression and significance of estrogen receptor(ER),progestogen receptor(PR),c-erbB-2 and nm23 protein in breast carcinoma. Methods 126 cases of surgical specimens from infiltrating breast cancer were investigated by S-P immunohistochemical staining for ER,PR,c-erbB-2 oncogene and nm23 gene protein. Results (1) The positive rate of ER was 44.4%,which was negatively correlated with histological grade,TNM stage and tumor size.The positive rate of ER in invasive lobular carcinoma was higher than that in ductal carcinoma(P<0.05),but that did not relate to age and metastasis;(2) The positive rate of PR was 63.5%,which was negatively correlated with histological grade,TNM stage,but did not relate to tumor size,histological types,age and metastasis;(3) The positive rate of c-erbB-2 was 61.9%.The expression of c-erbB-2 was positively correlated with histological grade,TNM stage,tumor size and lymph node metastasis,but did not relate to histological types and age;(4)The positive rate of nm23 was 71.4%,which was negatively correlated with histological grade,TNM stage and lymph node metastasis,and did not relate to tumor size and histological types;(5) The expression of ER was positively correlated with that of PR,so did nm23 with ER and PR,respectively,and that negatively correlated with c-erbB-2 expression.The expression of c-erbB-2 was negatively correlated with ER expression,but did not relate to PR expression. Conclusion The expression of ER,PR,c-erbB-2 and nm23 in breast cancer cells reflects the pathobiologic features of the cancer.