血管内皮生长因子与色素上皮衍生因子在糖尿病猕猴视网膜病变早期的表达

目的 检测糖尿病视网膜病变早期猕猴视网膜中血管内皮生长因子(VEGF)及色素上皮衍生因子(PEDF)的表达,探讨其意义.方法 通过空腹血糖值糖化血红蛋白水平、眼底彩照情况及糖尿病病程,确定入选3只利用高脂饲料诱导成功且处于糖尿病视网膜病变早期的猕猴及3只年龄相当的健康猕猴.行实时荧光定量PCR及免疫组织化学检测,观察猕猴视网膜内VEGF和PEDF表达情况.结果 处于糖尿病视网膜病变早期的猕猴视网膜内VEGF在mRNA表达水平(P＜0.05)及蛋白表达水平(P＜0.05)均明显高于对照组猕猴,糖尿病组猕猴视网膜内PEDF mRNA表达量相比对照组显著减少(P＜0.01),PEDF蛋白表达与其mRNA表达趋势一致(P＜0.05).结论 猕猴糖尿病视网膜早期,出现VEGF的上调和PEDF的下调,对糖尿病视网膜病变的发生具有一定的提示意义,可为早期糖尿病视网膜病变提供辅助诊断.

Expression of VEGF and PEDF in early-stage retinopathy in diabetic Macaca mulatta

Objective To explore the expression of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) in early stage of diabetic retinopathy (DR) in Macaca mulatta.Methods Three diabetic Macaca mulattas induced by high-fat diet were identified for early stage of diabetic retinopathy according to the fasting plasma glucose,hemoglobin Alc (HbA1c),fundus photograph and duration of diabetes,with another 3 age-matched healthy Macaca mulattas as control.The expression of VEGF and PEDF in the retinas of Macaca mulatta were detected by quantitative real-time PCR and immunohistochemistry.Result In early stage of diabetic retinopathy,VEGF mRNA and protein of the diabetic group were both significantly increased compared with the control group (P＜0.05).PEDF expression at both mRNA and protein levels was significantly decreased in diabetic Macaca mulattas compared with the control group (P＜0.01 and 0.05 respectively).Conclusion Retinal VEGF expression is increased and PEDF expression is decreased in early stage of diabetic retinopathy,suggesting their involvement in the occurrence of diabetic retinopathy and their value in assisting in the early diagnosis.