FSGS患者尿蛋白对肾小管上皮细胞活力及转分化的影响

目的：探讨局灶性-节段性肾小球硬化症（focal segmental glomerulosclerosis，FSGS）患者尿蛋白对肾小管上皮细胞（TECs）活力及转分化的影响。方法：用硫酸铵沉淀法提取FSGS患者尿液中的总蛋白成分，应用不同浓度尿蛋白处理体外培养人近端肾小管上皮细胞，然后应用全自动生化分析仪检测不同浓度尿蛋白刺激后HK-2细胞LDH值并计算其释放率、免疫细胞化学（ICC）法检测不同浓度尿蛋白刺激后HK-2细胞E-钙黏蛋白（e—cadherin）的表达水平和Western blotting法检测HK-2细胞α-平滑肌肌动蛋白（Q—SMA）蛋白表达水平。结果：所提取的尿蛋白主要成分为白蛋白、转铁蛋白和IgG等，分别占59．3％、15．3％和13．8％；肾小管上皮细胞LDH释放率和α—SMA蛋白的表达水平随尿蛋白浓度的升高而升高；E—cadherin的表达水平随着尿蛋白浓度的升高而下降。结论：FSGS尿蛋白可通过诱导肾小管上皮细胞损伤和转分化而促进肾小管-间质纤维化。

The Effects of Urine Protein from Patients with FSGS on Cell Vitality and Transdifferentiation in Renal Tubular Epithelial Cells

Objective:To explore the effects of urine protein from patients with FSGS on vitality and transdifferentiation in renal tubular epithelial cell(TECs).Methods:Urine protein was abstracted by fractional precipitation method with ammonium sulfate from nephrotic syndrome patients with FSGS.HK-2 cells were stimulated with urine protein in different concentration.The releasing rate of Lactic acid dehydrogenase(LDH)of HK-2 cells was measured by automatic biochemistry analyzer.The expression of E-cadherin in HK-2 c...