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| 锌指蛋白KLF4在K562细胞株中的表达和作用 | |
| 引用本文： | 张睿娟,杨林花. 锌指蛋白KLF4在K562细胞株中的表达和作用[J]. 白血病．淋巴瘤, 2009, 18(3): 137. DOI: 10.3760/cma.j.issn.1009-9921.2009.03.004 |
| 作者姓名： | 张睿娟 杨林花 |
| 作者单位： | 山西医科大学第二医院血液科,太原,030001;山西医科大学第二医院血液科,太原,030001 |
| 摘 要： | 目的 研究锌指蛋白KLF4在慢性粒细胞白血病细胞株K562中的表达，探讨KLF4在其中的作用。方法 采用RT-PCR及蛋白印迹法检测KLF4 mRNA、KLF4蛋白在K562细胞株和健康人单个核细胞中的表达。结果 KLF4在K562细胞株中表达，KLF4 mRNA表达量为0.02±0.01，与其在健康人单个核细胞中的表达0.43±0.03比较，差异有统计学意义。KLF4蛋白在K562细胞株中的表达也明显低于正常对照组。结论 KLF4在K562细胞株中呈低表达，提示KLF4可能具有肿瘤抑制因子作用，但其对白血病的增生、凋亡及耐药机制需要进一步研究。 |
| 关 键 词： | 蛋白质类 转录因子 白血病 K562细胞 |
| 收稿时间： | 2009-01-05; |
Effects and expression of KLF4 in leukemic cell line K562 |
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| ZHANG Rui-juan,YANG Lin-hua. Effects and expression of KLF4 in leukemic cell line K562[J]. Journal of Leukemia & Lymphoma, 2009, 18(3): 137. DOI: 10.3760/cma.j.issn.1009-9921.2009.03.004 | |
| Authors: | ZHANG Rui-juan YANG Lin-hua |
| Abstract: | Objective To investigate the expression and effect of zinc-finger protein kuppel-like factor4(KLF4) in leukemic cell line K562. Methods The lever of mRNA and protein of KLF4 is determined with real-time PCR and Western blot in human peripheral monoeyte/macrophage and K562 cells. Results Compared with control group (0.43±0.03), the expression of KLF4 mRNA in K562 cells (0.02±0.01) wassignificant different(P＜0,05). The expression of KLF4 protein in human peripheral monocyte macrophage is higher than that in K562 cells. Conclusion The low expression of KLF4 was present in leukemic cell line K562,which may suggest that KLF4 gene maybe a tumor inhibitor factor, and partially involved in leukemogenesis. |
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