凋亡相关蛋白在大肠癌中的表达及其与凋亡关系的研究

目的 探讨大肠癌中survivin和caspase-3的表达及其与细胞凋亡的关系.方法 采用免疫组织化学法测定50例正常大肠黏膜和66例大肠癌组织中survivin和caspase-3的表达.结果 survivin在大肠癌中的表达明显高于正常大肠黏膜组织(48.5%vs0,P<0.01),与淋巴结转移和分化程度有关(P<0.05).caspase-3在大肠癌中的表达明显低于正常组织(46.9%vs80.0%,P<0.05),与Dukes分期有关(P<0.05).survivin和caspase-3的表达具有相关性(P<0.05).结论 survivin、caspase-3与大肠肿瘤的细胞凋亡密切相关,参与细胞凋亡的调节机制,在大肠癌恶变的过程中发挥重要的作用.

Expression of apoptosis-related protein in colorectal carcinoma and its relationship with apoptosis

Objective To explore the expression of survivin and caspase-3 in colorectal carcinoma and its relations with tumor cell apoptosis.Methods The immunohistochemistry was used to detect the expression of survivin and caspase-3 in 66 cases of colorectal cancer tissues and 50 cases of normal tissues.Results Survivin was expressed in 32 of 66 cases(48.5%)of colorectal carcinoma,but not expressed in normal tissues.The expression of survivin was significantly correlated with lymph node metastasis and histological di...