胰岛素受体底物和酪氨酸磷酸酶在 2 型糖尿病大鼠肌肉组织中的蛋白表达及其意义

目的研究2型糖尿病对胰岛素产生抵抗的分子机制。方法用Western杂交检测2型糖尿病模型(OLETF大鼠)肌肉组织内IRS-1及SH-PTP2的蛋白表达水平,并以同种属的Wistar大鼠做比较。结果OLETF糖尿病大鼠的空腹和餐后血糖水平较对照组明显增高(P<0.05);Western杂交显示OLETF大鼠肌肉组织内IRS-1蛋白表达较对照组减少(P<0.05);而SH-PTP2的蛋白表达则较对照组增加(P<0.05)。结论IRS-1及SH-PTP2蛋白表达的异常改变,可能是引起2型糖尿病产生胰岛素抵抗的分子机制之一。

The Protein Expression of Insulin Receptor Substrate 1 and Protein Tyrosine Phosphatase with Two Src-homology 2 in the Skeletal Muscle Tissue of Type 2 Diabetic Rats

Objective To explore the molecular mechanisms of insulin-resistance of type 2 diabetes mellitus.Methods The levels of protein expression of insulin receptor substrate(IRS-1)and protein tyrosine phosphatase with two src-homdogy2(SH-PTP2)in skeletal muscle tissue of type 2 diabetic model(OLETF rats)were measured by Western blot analysis,and compared with those of congenerous Wistar rats.Results In OLETF diabetic rats,significant increase of blood glucose level appeared.The expression of IRS-1 protein had a significant reduction in the skeletal muscle tissue from OLETF rats than that of congenerous Wistar rats(P<0.05),but the protein expression of SH-PTP2 was significantly higher(P<0.05).Conclusions The abnormal changes in expression of IRS-1 and SH-PTP2 protein may be one of the mechanisms leading to insulin resistance of type 2 diabetes mellitus.