HPLC Fingerprint and LC-TOF-MS Analysis on Extract from Roots of Gentiana macrophylla |
| |
Authors: | SU Qi SHANG Ping-ping ZHANG Yong-min JIA N HE Jiao ZHAO Wen-na and SUN Wen-ji |
| |
Institution: | 1. Biomedicine Key Laboratory of Shaanxi Province,Northwest University,Xi'an 710069,China 2. Institut Parisien de Chimie Moléculaire,Université Pierre et Marie Curie-Paris 6,75005 Paris,France |
| |
Abstract: | Objective Establishing a fingerprint method to identify the characteristic chemicals in the roots of Gentiana macrophylla and evaluate their quality. Methods RP-HPLC was developed for fingerprint analysis and determination of four ingredients in G. macrophylla roots from different sources. LC-ESI-TOF-MS was employed to identify the chromatographic peaks of the fingerprint. Results Five common peaks were identified by comparing their retention time with reference secoiridoid glucosides. Eight major peaks in chromatographic fingerprint were analyzed by on-line LC-ESI-TOF-MS. Four secoiridoid glucosides were identified based on their MS data. Conclusion The method is specific and could be served for the quality identification and comprehensive evaluation of G. macrophylla. |
| |
Keywords: | fingerprint Gentiana macrophylla LC-ESI-TOF-MS quality control secoiridoid glucosides |
本文献已被 CNKI 万方数据 ScienceDirect 等数据库收录! |
| 点击此处可从《中草药(英文版)》浏览原始摘要信息 |
| 点击此处可从《中草药(英文版)》下载免费的PDF全文 |