大肠癌APE1的表达特点及临床意义

目的 探讨脱嘌呤／脱嘧啶核酸内切酶（APE1）在大肠癌发生、发展中的作用。方法 应用免疫组化SP法检测125例大肠癌、72例大肠腺瘤、60例癌旁大肠黏膜和40例正常大肠黏膜中APE1的表达情况，并分析APE1与大肠癌临床病理之间的关系。结果 正常大肠黏膜APE1呈胞核表达，大肠腺瘤和大肠癌组织APE1表达特征发生改变，呈胞核表达、单纯胞质表达或核浆共同表达。APE1胞质异位表达率大肠癌组织为73．6％，大肠腺瘤组织为83．3％，二者无显著性差异（P〉0．05），但均显著高于癌旁大肠黏膜（10％）和正常大肠黏膜（0％，P〈0．01）。APE1胞质异位表达与大肠癌临床分期和淋巴结转移有关（P〈0．01，P〈0．05）。结论 APE1胞质异位表达可能在大肠癌的发生、发展中起重要作用。

Expression feature of APE1 and its clinical implication in colorectal carcinoma

Objective To investigate the role of APE1 in the carcinogenesis and progression of colorectal carcinoma (CRC). Methods Expression of APE1 was determined with SP immunohistochemical technique in 40 specimens of normal colorectal mucosa, 60 specimens of colorectal mucosa adjacent to CRC, 72 specimens of colorectal adenoma, and 125 specimens of colorectal carcinoma. Results In normal colorectal mucosa, APE1 was detected in nuclei of epithelial cells. Shift of APE1 from nucleus to cytoplasm was observed in 6 of 60 (10%) specimens of mucosa adjacent to cancer. Such shift was observed in 92 of 125 (73.6%) CRC tissues and 60 of 72 (83.3%) colorectal adenoma, the incidence of both of them was significantly higher than that observed in normal colorectal mucosa and colorectal mucosa adjacent to CRC (P<0.01). Statistical analysis showed that the shift of APE1 from nucleus to cytoplasm was correlated with Dukes stage and lymph node metastasis in CRC(P<0.01,P<0.05). Conclusion The shift of APE1 from nucleus to cytoplasm might play a pivotal role in carcinogenesis and progression of colorectal carcinoma.