The effects of ALA-PDT on microbiota in pilosebaceous units of patients with severe acne: A metagenomic study |
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| Institution: | 1. Department of Dermatology, The First Affiliated Hospital of Soochow University, Suzhou, China;2. Department of Dermatology, The Affiliated Wuxi No. 2 People’s Hospital of Nanjing Medical University, Wuxi, China;3. Department of Cosmetic Laser Surgery, Institute of Dermatology, Peking Union College & Chinese Academy of Medical Sciences, Nanjing, China;1. Department of Ophthalmology, Health Sciences University, Tepecik Training and Research Hospital, Izmir, Turkey;2. Department of Ophthalmology, Dicle University Faculty of Medicine, Diyarbakır, Turkey;1. Department of Dental Materials and Prosthodontics, Araçatuba School of Dentistry, São Paulo State University - UNESP, Araçatuba, SP, Brazil;2. Department of Preventive and Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University - UNESP, Araçatuba, SP, Brazil;1. Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, China;2. Shanghai Key Laboratory of Gynecologic Oncology, Shanghai, 200127, China;3. State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiaotong University, China;1. São Leopoldo Mandic School of Dentistry, Dr. José Rocha Junqueira Street 13, Campinas, SP, Brazil;2. University Center of Patos de Minas, School of Dentistry, Avenida Maraba 831, Patos de Minas, MG, Brazil;3. Department of Pediatric Dentistry, University of São Paulo, Av. Professor Lineu Prestes, Cidade Universitária 2227, São Paulo, SP, Brazil;4. Universidade Católica Portuguesa, Faculdade de Medicina Dentária, Viseu, Portugal |
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| Abstract: | Background5-aminolevulinic acid mediated photodynamic therapy (ALA-PDT) is increasingly used to control severe acne. However, its impact on skin microbiota remains uncertain.ObjectivesWe aimed to compare the makeup, diversity, and function of the microbiota in pilosebaceous units of patients with severe acne before and after ALA-PDT.MethodsA longitudinal cohort study was performed on 11 participants with severe facial acne. All patients were given 5%ALA-PDT every two weeks for three sessions in total. The contents of lesions were sampled for metagenomic sequencing at baseline and two weeks after the first ALA-PDT session.ResultsCutibacterium acnes was the most dominant species followed by Staphylococcus epidermidis and Pseudomonas fluorescens. Treatment with ALA-PDT led to clinical improvements in acne severity concurrent with a significant reduction in the relative abundance of C. acnes, while P. fluorescens increased significantly after ALA-PDT. No significant change was identified in other species. ALA-PDT administration was associated with an increased microbiota diversity and reductions in the relative abundance of the functional genes involved in energy metabolism and DNA replication.ConclusionsALA-PDT plays a therapeutic role by killing C. acnes, increasing P. fluorescens and the microbiome diversity, while inhibiting the function of microbiota in pilosebaceous units of severe acne. |
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| Keywords: | Severe acne Microbiome ALA-PDT |
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