灵芝活性多糖GLIS对正常和荷瘤小鼠骨髓巨噬细胞的激活作用

为研究灵芝活性多糖GLIS对正常和荷瘤小鼠巨噬细胞的激活作用,用灵芝活性多糖GLIS刺激体外培养的正常和荷瘤小鼠的巨噬细胞,检测GLIS刺激后巨噬细胞分泌至培养基中的TNF-α、IL-1β和NO的含量,以及小鼠巨噬细胞对乳胶颗粒的吞噬率的变化,并检测GLIS刺激的巨噬细胞对肿瘤细胞的抑制作用,同时研究灵芝活性多糖GLIS组成的糖和蛋白部分对活性的影响。结果显示经灵芝活性多糖GLIS刺激后,能显著刺激巨噬细胞分泌TNF-α和IL-1β,并产生大量的NO。小鼠巨噬细胞对乳胶颗粒的吞噬功能也明显的增强,且荷瘤小鼠的巨噬细胞对GLIS的敏感度要优于正常小鼠,GLIS中的糖部分对它的活性作用起主要作用。该研究表明,灵芝活性多糖GLIS对正常和荷瘤小鼠巨噬细胞均具有明显的激活作用。

Activation of normal and tumor-bearing mouse macrophages by active polysaccharide from Ganoderma lucidum

To investigate the activation of normal and tumor,bearing mouse macrophages by the active polysaccharides from Ganoderma lucidum (GLIS), the mouse macrophages cultured in vitro were stimulated in presence of GLIS,contents of cytokines IL,1β,TNF,α and NO production in the culture supernatants were detected. The percentage of phagocytosis of latex beads by mouse macrophages was counted under microscope,the cytotoxicity of macrophages to tumor cells was also studied, and the composition of GLIS to the activity was investigated. The results showed that GLIS,activated mouse macrophages could secrete cytokines IL,1β and TNF,α and produce a great deal of NO. The percentage of phagocytosis of latex beads by mouse macrophages was also significantly increased in the presence of GLIS and it triggered macrophage activation for tumor cytotoxicity. This investigation indicate that GLIS can activate markedly the normal and tumor mouse macrophages.