| Study on the Relationship between Heat Shock Protein 70 and Toll-like Receptor-4 of Monocytes |
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| 作者姓名： | 陈新忠 孙宗全 杜心灵 刘超 柳祎 吴龙 |
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| 作者单位： | Department of Cardiovascular Surgery，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department of Cardiovascular Surgery，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department of Cardiovascular Surgery，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department of Cardiovascular Surgery，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department of Cardiovascular Surgery，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China，Department of Cardiovascular Surgery，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China |
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| 基金项目： | agrantfromtheNationalNaturalScienceFoundationofChina(No.30471715,C03030304) |
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| 摘 要： | TolllikereceptorsarethehumanhomologueoftheDrosophilaTollprotein.TheybelongtotheIL1receptorfamilycontainingrepeatedleucinerichmotifsintheirextracellularproteinandarelinkedtoasignalingpathwaythatinvolvestheIL1receptorassociatedkinaseandNFκB.Recentstudieshavesuggestedthatautologousheatshockprotein70(hsp70)servesasadangersignaltotheinnateimmunesystem1].Interestingly,microbialhsp70alsoinducesaproinflammatoryresponseininnateimmunecells,suggestingthatdamagedautologouscellsandmincrobialpathogens…
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| 关 键 词： | 热休克蛋白70 长途表达受体-4 单核细胞 炎症 TNF-α |
| 收稿时间： | 23 July 2010 |
Study on the relationship between heat shock protein 70 and toll-like receptor-4 of monocytes |
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| Xinzhong,Chen,Zongquan,Sun,Xinling,Du,Chao,Liu,Yi,Liu,Long,Wu.Study on the Relationship between Heat Shock Protein 70 and Toll-like Receptor-4 of Monocytes[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2004,24(6):560-562. |
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| Authors: | Xinzhong Chen Zongquan Sun Xinling Du Chao Liu Yi Liu Long Wu |
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| Institution: | Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | To explore the relation between human heat shock protein 70 (hsp70) and TLR4 in human monocytes in vitro, human monocytes were stimulated with various concentrations of HSP70, and TNF-alpha production in supernatants was measured by ELISA. Pre-incubated with or without anti-TLR4 mAb, and stimulated with hsp70 (5.0 microg/ml), NF-kappaB p65 of human monocytes in different time points were detected by immunohistochemistry and monocyte surface expression of TLR4 was measured by flow cytometry. After the human monocytes were pre-incubated with various concentrations of anti-TLR4 and stimulated with hsp70 (5.0 microg/ml), TNF-alpha production in supernatants was measured. The results showed that hsp70 enhanced NF-kappaB activation, which was clearly inhibited by anti-TLR4, with the positive cell ratios being 67.44%, 39.17%, 31.56% and 28.05 %, respectively. TLR4 was rapidly down-regulated in the presence of hsp70. MFI of TLR4 on monocytes in different time points were 87.77 +/- 5.38, 78.16 +/- 6.01 and 45.17 +/- 4.97 (P<0.05), 26.98 +/- 5.83 (P<0.01), respectively. Moreover, hsp70-induced TNF-alpha production by human monocytes was inhibited by anti-TLR4. It is suggested that TLR4 is involved in the hsp70-mediated activation of innate immunity. |
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| Keywords: | heat shock proteins Toll-like receptors inflammation monocyte |
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