枳实对大鼠小肠电活动影响的下丘脑作用机制研究

目的观察枳实对在体大鼠小肠电活动的影响,探讨其作用与下丘脑内脑肠肽胆囊收缩素(CCK)、生长抑素(SS)的相关性。方法采用消化道腔外电极记录法观察枳实组与生理盐水组移行性综合肌电(MMC)周期时程、活动期/周期比例、活动期每分钟快波数的变化；采用免疫组织化学法结合显微图像定量分析扫描系统检测下丘脑背内侧核(DMH)、腹内侧核(VMH)、下丘脑外侧区(LHA)CCK的相对含量,以及下丘脑VMH、LHA、室旁核(PVN)SS的相对含量。结果枳实组与生理盐水组比较MMC周期缩短、活动期/周期比例增大、活动期每分钟快波数增加,差异有显著性；与生理盐水组及空白对照组比较枳实组DMH、VMH、LHA的CCK阳性神经元表达降低；VMH、LHA、PVN的SS阳性神经元表达增高,与生理盐水组及空白对照组比较差异有显著性。结论枳实对大鼠小肠电活动有兴奋作用,这种兴奋作用可能与下丘脑内的CCK、SS有关。

Mechanism of Hypothalamic Effect in Small Intestine Electro-activity of Rats Regulated by Fructus Aurantii Immaturus

Objective To observe the effect of Fructus Aurantii Immaturus(FAI)on the electro-activity of small intestines in rats,and evaluate the interrelations between the FAI regulating effect and choecystokinin (CCK)and somatostatin(SS).Methods Migrating myoelectric complex(MMC)cyclic period,the ratio of the active time to the cyclic period,and the number of the fast wave within the active time per minute were observed between FAI and the normal saline group by external alimentary canal electrodes;the CCK contents in dorsomedial hypothalamic nucleus(DMH),ventromedia hypothalamic nucleus(VMH),lateral hypothalamus area(LHA)and SS in VMH,LHA,paraventricular nucleus(PVN)by using immuno-chemistry technique and micro-image pattern quantitative analysis and scanning system.Results The MMC cyclic period shortened,the ratio of the active time to the cyclic period increased and the number of the fast wave within the active time per minute increased in the FAI group,which showed significant difference from the normal saline group;CCK positive neurons were reduced in the areas of DMH,VMH and LHA,SS positive neurons were increased in the areas of VMH,LHA and PVN in the FAI gioup,which showed significant difference compared with the normal saline and the blank control group. Conclusion FAI can stimulate the electro-reactivity of small intestines.The stimulative effect of FAI might be re- lated to CCK and SS in hypothamus.