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| 白细胞介素6在病毒性心肌炎发病中的作用 | |
| 引用本文： | 刘亚黎,尾内善四郎. 白细胞介素6在病毒性心肌炎发病中的作用[J]. 中华儿科杂志, 1999, 0(2): 101-103 |
| 作者姓名： | 刘亚黎 尾内善四郎 |
| 作者单位： | [1]同济医科大学附属协和医院小儿科 [2]日本京都府立医科大学小儿疾病研究施设内科部门 |
| 摘 要： | 目的 探讨白细胞介素６（ＩＬ－６）在病毒性心肌炎发病中的作用。方法 用脑心肌炎病毒（ＥＭＣＶ）诱发小鼠急性心肌炎，使用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）及ＥＬＩＳＡ法测定了心肌组织内ＩＬ－６ｍＲＮＡ的表达及蛋白含量的变化，使用抗ＩＬ－６抗体体现人投入观察其作用。结果 ＥＭＣＶ感染后ＩＬ－６ｍＲＮＡ的表达明显增强，蛋白含量增加．抗ＩＬ－６抗体投入后导致生存率降低（Ｐ〈０．０５），心肌内病毒滴定度增高 |
| 关 键 词： | 白细胞介素6 心肌炎 病毒性心肌病 |
Effect of anti interleukin 6 antibody on murine acute viral myocarditis |
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| LIU Yali Onouchi Zenshiro. Effect of anti interleukin 6 antibody on murine acute viral myocarditis[J]. Zhonghua Er Ke Za Zhi, 1999, 0(2): 101-103 | |
| Authors: | LIU Yali Onouchi Zenshiro |
| Affiliation: | LIU Yali * Onouchi Zenshiro. * Department of Pediatrics,Xiehe Hospital,Tongji Medical University,Wuhan,430022 |
| Abstract: | Objective To investigate the role of interleukin 6 (IL 6) in viral myocarditis. Methods The encephalomyocarditis virus (EMCV) was used to induce acute myocarditis in mice. The following experiments were performed: (1) detections of IL 6 mRNA level expression in cardiac muscle cells after EMCV infection by the RT PCR method and IL 6 concentration in cardiac muscle cells by ELISA; (2) the effect of the anti IL 6 antibody in vivo. Results Enhanced expression of IL 6 mRNA and IL 6 concentration were observed in cardiac muscle cells during the acute stage after EMCV infection. In addition, treatment with anti IL 6 antibody resulted in a significantly reduced survival ( P <0.05), increased myocardial virus titers ( P <0.05), augmented inflammation and myocardial damage ( P <0.05). Conclusion IL 6 may play an important role as protective factor in the acute viral myocarditis. |
| Keywords: | Interleukin 6 Myocarditis Virus diseases |
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