急性间断低氧大鼠脑细胞程序性死亡与自由基消除能力的关系

为探讨高原低氧神经细胞细胞性死亡的机制，利用急性间断低氧动物模型，用ＴＵＮＥＬ法检测了脑细胞的程序性死亡，化学法制定组织中ＧＳＨ／ＧＳＳＧ比值和ＳＯＤ活性。结果表明，随着低氧时间延长，组织中ＧＳＨ／ＧＳＳＧ比值与ＳＯＤ活性下降；而程序性死亡细胞数目增加，相关与回归分析表明，程序性死亡细胞数与ＧＳＨ／ＧＳＳＧ比值和ＳＯＤ活性成负的幂相关，提示，程序性细胞死亡与自由基消除系统可能存在内的联系。

Relationship between apoptosis of brain cells and free radical scavenging ability in rats during acute intermittent hypoxia.

It has been found that plateau hypoxia could induce apoptosis in rats' brain, but the mechanism was not clear. In order to study it further, we utilized acute intermittent hypoxia model to investigate reduced gliutathione (GSH), oxidized gliutathione(GSSG), super-oxide dismutase (SOD) and programmed cell death (PCD) or apoptosis (AP) in rats' brain. The results showed that with the increase of hypoxic exposure duration, apoptotic cells increased, the ratio of GSH/GSSG and the activity of SOD dropped. Regression analysis showed that there was a negative power relationship between the number of apoptotic cells and the ratio of GSH/GSSG or between the number of apoptotic cells and the activity of SOD in a limited range. It was concluded that there would be an intrinsic relationship between apoptosis and free radical scavenging ability in the organ.