脂氧酶调节对大鼠肝癌前病变的影响

目的 观察脂氧酶调节的抑制剂-槲皮素（QU）在肝癌发生过程，即癌前病灶转化为增生结节过程中的作用，探讨脂氧酶调节对肝癌发生的影响。方法利用大白鼠复制肝癌前病变发生的动物模型，然后将带有癌前病灶的大鼠分组。实验组分别给予0．75％，1．5％槲皮素，加或不加0．05％苯巴比妥（PB）的饲料，对照组只给予单纯0．05％PB饲料或基础饲料喂养9周。处死取肝后，进行r-谷胺酰转换酶（GGT）组化染色和HE染色。结果0．75％。1．5％槲皮素，加或不加PB的实验组中，单位面积的GGT染色阳性的持续性结节数和HE染色的肝细胞增生性结节数与相应对照组相比显著减少（P〈0．05）。结论2种浓度的槲皮素在有或没有PB-癌促进剂作用下，均对肝癌前病灶转化增生结节的过程有抑制作用，提示脂氧酶调节通路与肝癌的发生有关。

Effects of lipoxygenase pathway on preneoplastic lesions in rat liver

Objective To investigate effects of lipoxygenase pathway on hepatocarcinogenesis.And quercetin(QU),one of inhibitors of lipoxygenase pathway,was observed on procedure from preneoplastic foci into nodules in rat liver.Methods Inducing preneoplastic liver foci,then rats bearing foci were given diets containing 0.75%,1.5% QU with or without 0.05% Phenobarbital(PB),0.05% PB alone and basal diet for 9 weeks.GGT staining and HE staining were made in liver slices.Results Both doses QU either with or without PB accelerated the remodeling of preneoplastic foci,significantly decreased the numbers of persistent nodules and hyperplastic nodules.Conclusion Both doses QU inhibited the evolution of preneoplastic foci into nodules in rat liver with or without PB exposure,suggesting that lipoxygenase pathway involved in hepatocarcinogenesis in rats.