| FDP对微波辐射后大鼠海马COX表达的影响 |
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| 引用本文： | 赵黎,彭瑞云,高亚兵,王水明,王晓民,徐新萍,王丽峰,董霁,杨国山.FDP对微波辐射后大鼠海马COX表达的影响[J].中华放射医学与防护杂志,2008,28(4):421-423. |
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| 作者姓名： | 赵黎 彭瑞云 高亚兵 王水明 王晓民 徐新萍 王丽峰 董霁 杨国山 |
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| 作者单位： | 1. 军事医学科学院放射与辐射医学研究所,北京,100850
2. Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
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| 基金项目： | 军队医药卫生专项基金 |
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| 摘 要： | 目的探讨1, 6-二磷酸果糖(FDP)对微波辐射后大鼠海马线粒体呼吸链相关基因细胞色素c氧化酶(cytochrome c oxidase,COX)表达的影响。方法Wistar雄性大鼠随机分为假辐射组(5只),辐射组(10只)和辐射+药物组(10只)。采用30mW/cm2微波辐射,辐射+药物组于辐射前3d 腹腔注射350mg/kg FDP,连续给药3或10d,1次/d。于辐射后6h和7d活杀取海马,采用反转录-聚合酶链反应(RT-PCR)和图像分析检测大鼠海马组织COX亚基Ⅰ、Ⅱ和Ⅳ mRNA表达,通过Western blot和图像分析检测大鼠海马组织COX Ⅰ蛋白表达变化。结果辐射后6h和7d大鼠海马COX Ⅰ、Ⅱ和Ⅳ mRNA表达均较假辐射组降低,药物组大鼠海马COX Ⅰ、Ⅱ和Ⅳ mRNA与相应辐射组相比均见表达增加,以给药后3d(辐射后6h)组增加最为显著(tCOX Ⅰ=3.2205,tCOX Ⅱ=3.5762,tCOX Ⅳ=3.0408,P<0.05)。辐射后6h和7d大鼠海马COX Ⅰ蛋白表达均较假辐射组减少,给药后3d(辐射后6h)与辐射后6h相比COX Ⅰ蛋白表达升高(t=2.9614,P<0.05),给药后10d(辐射后7d)与辐射后7d相比改变不显著。结论FDP对微波辐射后大鼠海马线粒体呼吸链相关基因COX表达降低有一定的促恢复作用。
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| 关 键 词： | 微波辐射 大鼠 海马 线粒体 |
| 收稿时间： | 2008/2/28 0:00:00 |
Effects of fructose-1, 6-diphosphate on COX expression of mitochondrial respiratory chain in rats' hippocampus after microwave exposure |
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| ZHAO Li,PENG Rui-yun,GAO Ya-bing.Effects of fructose-1, 6-diphosphate on COX expression of mitochondrial respiratory chain in rats' hippocampus after microwave exposure[J].Chinese Journal of Radiological Medicine and Protection,2008,28(4):421-423. |
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| Authors: | ZHAO Li PENG Rui-yun GAO Ya-bing |
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| Institution: | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract: | |
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| Keywords: | FDP COX |
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