受体大鼠肠道缺血预处理对肠道黏膜屏障及移植肝脏的影响

目的探讨肠道缺血预处理(IPC)对肠道黏膜屏障的作用及其对大鼠移植肝脏形态和功能的影响。方法清洁级SD大鼠,随机分为3组:S组为假手术组(n=8);A组为肝脏移植组(n=20);B组为肠道缺血预处理肝脏移植组(n=20)。A,B组在移植后1h各取8只,测定其肠道MPO与MDA含量,血清SOD,NO含量及肝功能各项指标;光镜下观察小肠组织病理改变;测定黏膜湿重、微绒毛高度和宽度及观察移植肝脏肝小叶形态结构的变化,余动物观察存活率。结果 B组1 d生存率与A组比较无统计学差异(P0.05),但1周生存率有显著提高(P0.05)。A组小肠MPO活性与MDA含量明显高于S组和B组,血清SOD活性和NO含量则显著减低,空肠黏膜湿重、绒毛高度和宽度显著低于S组与B组(均P0.01)。光镜观察见A组受鼠空肠与移植肝脏的形态学损伤性改变较B组与S组为重。结论受体大鼠肠道缺血预处理对大鼠移植肝脏具有保护作用。

The effect of ischemic preconditioning of the small intestine on intestinal mucosal barrier and liver graft in rats

Objective:To investigate the effect of ischemic preconditioning of the small intestine on intestinal mucosal barrier and liver graft in rats.
Methods:SD rats were randomly assigned into the following study groups: group S (sham operation group, n=8), group A (orthotropic liver transplantation group, n=20), group B (ischemic preconditioning on the recipient rat′s small intestine, n=20). At postgraft 1 h, each 8 rats were obtained from groups A and B. The MDA and MPO of the intestine,as well the serum SOD and NO and liver function were determined. The morphological changes of liver lobules and the epithelial basement membrane and mucosa of small intestine were observed by microscopy.
Results:The one-day-survival between in groups A and B was not significant. The one-week-survival in group B  was significantly increased than that in group A. MPO and MDA in group A was significantly higher than in group B and group S; SOD and NO in group A were higher than those in group B and group S. The mucosal wet weight, villus height and villus wide in group A were significantly lower than those in  group B and group S.The lesions of hepatic cells and intestine unber microscope were milder in group B than that in group A.
Conclusions:Ischemic preconditioning of the recipient rat small intestine has early protective effect on liver graft.