| 克拉霉素/氟罗沙星对铜绿假单胞菌生物被膜体外作用研究 |
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| 引用本文： | 李颢,王亚,张晓举,王志东,胡贺敏,程金玲.克拉霉素/氟罗沙星对铜绿假单胞菌生物被膜体外作用研究[J].中国药房,2003,14(7):398-399. |
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| 作者姓名： | 李颢 王亚 张晓举 王志东 胡贺敏 程金玲 |
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| 作者单位： | 河南省汝南县人民医院,汝南县,463300 |
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| 摘 要： | 目的 :探讨克拉霉素和氟罗沙星对铜绿假单胞菌 (PA)生物被膜的影响。方法 :选取临床分离的呼吸道PA7株 ,用平板法培养细菌生物被膜 ,银染色法鉴定 ;微量稀释法检测克拉霉素和氟罗沙星对PA的最低抑菌浓度(MIC) ;噻唑兰法测定活菌数 ;结晶紫染色法观察不同浓度药物对PA粘附性的影响。结果 :1/16MIC、1/4MIC克拉霉素可减少细菌对硅胶膜粘附(P<0 05) ,且可显著增强1/4MIC、1/2MIC的氟罗沙星对铜绿假单胞菌生物被膜的抑菌活性 (F=20 193 ,P<0 01)。结论 :克拉霉素与氟罗沙星联合应用对生物被膜铜绿假单胞菌呈抗菌协同作用
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| 关 键 词： | 铜绿假单胞菌 生物被膜 克拉霉素 氟罗沙星 |
| 文章编号： | 1001-0408(2003)07-0398-02 |
| 修稿时间： | 2002年4月23日 |
Effects of Clarithromycin and Fleroxacin on Pseudomonas Aeruginosa Biofilm in Vitro |
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| LI Hao,WANG Ya,ZHANG Xiaoju,WANG Zhidong,HU Hemin,CHENG Jinling.Effects of Clarithromycin and Fleroxacin on Pseudomonas Aeruginosa Biofilm in Vitro[J].China Pharmacy,2003,14(7):398-399. |
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| Authors: | LI Hao WANG Ya ZHANG Xiaoju WANG Zhidong HU Hemin CHENG Jinling |
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| Abstract: | OBJECTIVE:To observe the effects of clarithromycin and fleroxacin on Pseudomonas aeruginosa(PA) biofilm METHODS:Clinical isolates of 7 strains of PA from respiratory tract were cultured with modified plate culture method;bacterial biofilm model was identified by silver nitrate staining;MICs were determined by broth microdilution The number of viable bacteria in biofilm was measured by using MTT method and bacterial adherence was measured by crystal violet staining RESULTS:1/16MIC and 1/4MIC of clarithromycin could inhibit the adherence of PA to silica-gel film(P<0 05)and significantly enhance the bacteriostatic effect of 1/4MIC and 1/2MIC of fleroxacin on PA in biofilm(F=20 193,P<0 01) CONCLUSION:There are synergetic effects against biofilm PA when clarithromycin is combined with fleroxacin in vitro |
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| Keywords: | Pseudomonas aeruginosa biofilm clarithromycin fleroxacin |
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