液态氮冷冻保存对异种神经移植后神经再生的影响

将成年日本大耳兔的胫神经在液态氮中保存４周或４０周，经反复冰冻、融解５次后移植至Ｗｉｓｔａｒ大白鼠的坐骨神经中。异种神经移植术后８、１２、２４周可观察到宿主的再生神经纤维从近段坐骨神经越过近端吻合部长入异种移植神经，继而越过远端吻合部向远段坐骨神经延伸。用体视学方法对异种神经移植段再生神经纤维的ＡＡ、ＮＡ和ＡＡ／ＮＡ测定和比较表明：异种神经在液态氮中保存４周和４０周后再移植，不影响宿主神经纤维的再生，两组之间的再生神经纤维无显著性差别

The Effect of Freezing Preservation with Liquid Nitrogen on Nerve Regeneration in Heterograft Nerve

Adult Japanese big ear rabbit's tibial nerve,preserved in liquid nitrogen respectively for 4 weeks and 40 weeks,was grafted into the sciatic nerve of Wistar rat by repeated freezing and thawing for 5 times.The regenerating nerve fibers of the host crossed the proximal suture into the heterograft nerve,passed through the distal suture and extended into the distal sciatic nerve.The regenerating nerve fibers of the heterograft nerve segment were measured by the method of steroscopy.The result indicated that the heterograft nerve preserved in liquid nitrogen for 4 weeks or 40 weeks had no effect on nerve regeneration of the host nerve fiber,nor was there any significant difference between the regenerating nerve fibers of the two groups.