三叶青提取物对肺癌A549细胞的体外抑制作用

目的:探讨三叶青提取物对肺癌细胞A549的体外抑制作用。方法:采用体外细胞培养技术,用MTT法观察三叶青提取物对肺癌细胞A549体外抑制作用的时效、量效关系;琼脂糖凝胶电泳检测细胞凋亡;RT-PCR法检测细胞凋亡相关基因BcL-2的表达。结果:三叶青提取物以1×10-3g·L-1的浓度与A549细胞共培养48 h,其细胞活性有显著降低;不同浓度的三叶青提取物和A549细胞共同培养,药物浓度为1×10-2、1×10-31、×10-4g·L-1的三叶青提取物对A549细胞活性有显著性抑制作用。琼脂糖凝胶电泳有明显的梯状条带;不同浓度三叶青提取物作用于A549细胞后,细胞BcL-2基因的表达随浓度的增加而降低。结论:三叶青提取物对A549细胞具有明显的体外抑制作用,其抑制作用与药物的浓度和作用时间呈明显正相关。

Depressant Efects of the Extract of Radix Tetrastigma Hemsleyani On Lung Carcinoma Cell Line A549 in vitro

Objective:To study depressant efects of the extract of Radix Tetrastigma Hemsleyani on lung carcinoma cell line A549 in vitro.Methods:The time-effect and quality-effect of the extract of Radix Tetrastigma Hemsleyani on lung carcinoma cell line A549 were observed by cell culturein vitro using the method of MTT.Agarose gel electrophoresis was used to detect the apoptosis.RT-PCR were used to detect the expression of BcL2 gene,which was related to apoptosis.Results:48 hours after co-culturing A549 and Radix Tetrastigma Hemsleyani with a concentration of 1×10^-3 g·L^-1.the cell activity decreased significantly compared to control.When culturing A549 and the extract with different concentrations,it had a significant depressant effect on cell activity with concentrations of 1×10^{-2,1×10-3,1×10-4 g·L-1.There was an obvious apoptotic ladder in agarose gel electrophoresis.Culturing A549 and the extract with different concentrations,the expression of BcL-2 gene was low following the density raise of Radix Tetrastigma Hemsleyani.Conclusions:the extract of Radix Tetrastigma Hemsleyani has a significant depressant effect on lung carcinoma cell line A549,which positively relates with its concentration and efect-time.}