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| HPLC法测定栀柏合剂中栀子苷的含量 | |
| 引用本文： | 孙文武. HPLC法测定栀柏合剂中栀子苷的含量[J]. 中国药房, 2007, 18(33): 2609-2610 |
| 作者姓名： | 孙文武 |
| 作者单位： | 湖北天门市第一人民医院药剂科,天门市,431700 |
| 摘 要： | 目的:建立以高效液相色谱法测定栀柏合剂中栀子苷含量的方法。方法:色谱柱为Shim-pack VP-ODS C18(150mm×4.6mm,5μm),流动相为乙腈-水(12∶88),流速为1mL.min-1,检测波长为238nm,柱温为25℃。结果:栀子苷检测浓度在25~100mg.L-1范围内与峰面积积分值呈良好线性关系(r=0.9997);平均回收率为99.89%,RSD=0.80%(n=9)。结论:本方法灵敏度高、专属性强、重现性好,可用于本制剂的质量控制。 |
| 关 键 词： | 高效液相色谱法 栀柏合剂 栀子苷 含量测定 |
| 文章编号： | 1001-0408（2007）33-2609-02 |
| 收稿时间： | 2006-12-04 |
| 修稿时间： | 2007-03-13 |
Quantitative Determination of Geniposide in Zhibo Mixture by HPLC |
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| SUN Wenwu. Quantitative Determination of Geniposide in Zhibo Mixture by HPLC[J]. China Pharmacy, 2007, 18(33): 2609-2610 | |
| Authors: | SUN Wenwu |
| Abstract: | OBJECTIVE:To establish a HPLC method for the quantitative determination of Geniposide in Zhibo mixture.METHODS:The separation of sample was performed on Shim-pack VP-ODS C18 column(150mm×4.6mm,5μm)at a temperature of 25℃.The mobile phase consisted of acetonitrile-water(12∶88)at a flow rate of 1mL·min-1.The detection wavelength was set at 238nm.RESULTS:The linear range of Geniposide was 25～100mg·L-1(r=0.999 7).The average recovery was 99.89%(RSD=0.80%,n=9).CONCLUSION:The method was proved to be sensitive,specific,reproducible and suitable for quality control of Zhibo mixture. |
| Keywords: | HPLC Zhibo mixture Geniposide Content determination |
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