亚洲地区肺炎克雷伯菌耐药基因和毒力基因分析

目的:分析亚洲地区肺炎克雷伯菌毒力基因和耐药基因的携带情况,为制定菌株防治措施提供依据。方法:从GenBank中收集并分析了4 549株肺炎克雷伯菌的完整基因组(下载日期:2020年12月31日)。通过MLST、Kleborate和ABRicate等生物信息学软件分析肺炎克雷伯菌的MLST分型、KL分型、毒力基因以及耐药基因。结果:MLST分型结果显示,ST11是亚洲地区肺炎克雷伯菌的主要序列类型,共检测到1 519(33.39%)株,其中ST11-KL64、ST11-KL47,分别占51.15%(777/1 519)、37.20%(565/1 519)。毒力基因检测发现,ST11-KL64型菌株中,有36.04%(280/777)的菌株同时携带4种铁载体基因,有33.33%(259/777)的菌株同时携带rmpA和rmpA2基因。在ST11-KL47型菌株中,有13.52%(76/565)的菌株同时携带4种铁载体基因,有5.49%(31/565)的菌株同时携带rmpA和rmpA2基因。耐药基因检测发现,有97.25%(4 424/4 549)的菌株检测出β-内酰胺类耐药基因,有3.06%(139/4 549)的菌株携带粘菌素耐药基因。结论:亚洲地区肺炎克雷伯菌MLST分型以ST11为主、血清荚膜型以KL64、KL47为主。ST11-KL64型菌株的高毒力表型基因携带率高于ST11-KL47。应规范抗菌药物使用,加强对肺炎克雷伯菌的监测和医院内感染的防控工作,防止肺炎克雷伯菌的大规模流行。

Analysis of drug resistance genes and virulence genes of Klebsiella pneumoniae in Asia

OBJECTIVE To analyze the virulence and resistance genes carrying by Klebsiella pneumoniae in Asia, and provide basis for the development of bacterial control measures.METHODS The complete genomes of 4,549 Klebsiella pneumoniae strains were collected and analyzed from GenBank (download date: December 31, 2020). The MLST, KL, virulence genes and resistance genes of Klebsiella pneumoniae were analyzed by bioinformatics software such as MLST, Kleborate and ABRicate.RESULTS The MLST classification showed that ST11 Klebsiella pneumoniae was the main sequence type in Asia, and 1 519 (33.39%) strains were detected, including ST11-KL64 (51.15%, 777/1 519) and ST11-KL47 37.20% (565/1 519). In addition, virulence genes detection found that 36.04% (280/777) of ST11-KL64 strains carried four siderophore genes at the same time, and 33.33% (259/777) of strains carried both rmpA and rmpA2 genes. Furthermore, in ST11-KL47, 13.52% (76/565) of the strains carried four siderophore genes, and 5.49% (31/565) of the strains carried both rmpA and rmpA2 genes. Antibiotic resistance genes were detected in 97.25% (4 424/4 549) of the strains, and 3.06% (139/4 549) of the strains had colistin resistance genes.CONCLUSION Here, our data show that the MLST type of Klebsiella pneumoniae in Asia is mainly ST11, and the serum capsular types are mainly KL64 and KL47. Additionally, the high virulence phenotype gene carrying rate of ST11-KL64 strain was higher than that of ST11-KL47. As described above, the use of antibiotics should be regulated, the monitoring of Klebsiella pneumoniae and the prevention and control of nosocomial infections should be strengthened to prevent the large-scale epidemic of Klebsiella pneumoniae.