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新型生物活性玻璃促进人工牙本质龋再矿化的作用
引用本文:郭若兰,黄桂彬,龙赟子,董艳梅.新型生物活性玻璃促进人工牙本质龋再矿化的作用[J].北京大学学报(医学版),2023,55(1):82-87.
作者姓名:郭若兰  黄桂彬  龙赟子  董艳梅
作者单位:北京大学口腔医学院·口腔医院牙体牙髓科,国家口腔医学中心,国家口腔疾病临床医学研究中心,口腔生物材料和数字诊疗装备国家工程研究中心,口腔数字医学北京市重点实验室,国家卫生健康委员会口腔医学计算机应用工程技术研究中心,国家药品监督管理局口腔生物材料重点实验室,北京 100081
基金项目:国家自然科学基金(81870753);国家自然科学基金(51372005)
摘    要:目的:研究以植酸为前驱体合成的生物活性玻璃(phytic acid derived bioactive CaO-P2O5-SiO2 gel-glasses, PSC)和含氟生物活性玻璃(fluoride-containing bioactive glasses, FBG)这两种新型生物活性玻璃(bioactive glass, BG)对人工牙本质龋再矿化的作用,为促进牙本质龋再矿化寻找更有效的方法。方法:(1)PSC是以植酸为磷前驱体、采用溶胶凝胶法制备,其化学组成为10.8%P2O5-54.2%SiO2-35.0%CaO(摩尔百分比); FBG采用熔融法制备,化学组成为6.1%P2O5-37.0%SiO2-53.9%CaO-3.0%CaF2(摩尔百分比);传统生物活性玻璃45S5采用熔融法制备,化学组成为6.0%P2O5-4...

关 键 词:生物活性玻璃  人工牙本质龋  脱矿  再矿化
收稿时间:2020-06-08

Effects of novel bioactive glasses on promoting remineralization of artificial dentin caries
Ruo-lan GUO,Gui-bin HUANG,Yun-zi LONG,Yan-mei DONG.Effects of novel bioactive glasses on promoting remineralization of artificial dentin caries[J].Journal of Peking University:Health Sciences,2023,55(1):82-87.
Authors:Ruo-lan GUO  Gui-bin HUANG  Yun-zi LONG  Yan-mei DONG
Institution:Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & NHC Research Center of Engineering and Technology for Computerized Dentistry & NMPA Key Laboratory for Dental Materials, Beijing 100081, China
Abstract:Objective: To investigate the effects of novel bioactive glasses (BG) including PSC with high phosphorus component and FBG with fluorine-doped element on promoting remineralization of artificial dentin caries. Methods: (1) BGs were used in this study as follows: PSC (10.8%P2O5-54.2%SiO2-35.0%CaO, mol.%) were synthesized using phytic acid as the phosphorus precursor through sol-gel method. FBG (6.1%P2O5-37.0%SiO2-53.9%CaO-3.0%CaF2, mol.%) and 45S5(6.0%P2O5-45.0%SiO2-24.5%CaO-24.5%Na2O, mol.%) were synthesized by traditional melt method. (2) The above BGs were soaked in simulated body fluid (SBF) for 24 hours. Then X-ray diffraction (XRD) was used to analyze the formation of hydroxyapatite (HA) crystals. (3) Prepared 1 mm thick dentin slices were soaked in 17% ethylene diamine tetraacetic acid (EDTA) for 1 week to demineralize the dentin. Then the dentin slices treated by BG were soaked in SBF for 1 week. Field emission scanning electron micro-scopy (FE-SEM) was used to observe the surface morphology of the dentin slices. (4) Four cavities were prepared to 1 mm depth in each 2 mm thick dentin slice, then were treated with lactic acid for 2 weeks to form the artificial dentin caries. Wax, mineral trioxide aggregate (MTA), PSC and FBG were used to fill four cavities as blank control group, MTA group, PSC group and FBG group respectively. Then the spe-cimens were soaked in SBF for 4 weeks. The changes of depth and density of demineralized dentin were analyzed using Micro-CT before filling and after 2 and 4 weeks filling. Results: (1) PSC and FBG promoted mineral formation on the surfaces of the demineralized dentin. And the speed was faster and crystallinity was higher in PSC group than the FBG and 45S5 groups. (2) The increased mineral density of artificial dentin caries in PSC group were (185.98 ± 55.66) mg/cm3 and (213.64 ± 36.01) mg/cm3 2 and 4 weeks after filling respectively, which were significantly higher than the control group (20.38 ± 7.55) mg/cm3, P=0.006; (36.46 ± 10.79) mg/cm3, P=0.001]. At meanwhile, PSC group was also higher than MTA group (57.29 ± 10.09) mg/cm3; (111.02 ± 22.06) mg/cm3], and it had statistical difference (P=0.015; P=0.006). The depth of remineralized dentin in PSC group were (40.0 ± 16.9) μm and (54.5 ± 17.8) μm 2 and 4 weeks respectively, which were also statistically different from the control group (P =0.010;P=0.001). There were no statistical differences between the control group and MTA group. The above effects of FBG group were between PSC and MTA. Conclusion: PSC has advantages in the speed, quality and depth of mineral deposition in the demineralized layer of artificial dentin caries. It would be expected to be an ideal material to promote the remineralization of dentin caries.
Keywords:Bioactive glass  Artificial dentin caries  Demineralization  Remineralization  
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