超高效液相色谱法同时测定中草药保健食品中7种功效成分

目的建立灵敏、简便的中草药保健食品中7种功效成分(丹参酮ⅡA、芍药苷、栀子苷、丹皮酚、芦丁、淫羊藿苷和木兰花碱)同时测定的超高效液相色谱方法。方法样品用60%甲醇超声提取,采用C18色谱柱分离,以甲醇—0.1%甲酸水溶液为流动相,梯度洗脱,紫外波长检测,外标法定量。结果丹参酮IIA、芍药苷、栀子苷、芦丁、木兰花碱在0.50~50.0 mg/L,丹皮酚和淫羊藿苷在0.10~25.0 mg/L范围内有良好的线性关系,相关系数r≥0.9992。方法检出限为0.1~1 mg/kg,回收率88.9%~105.0%,相对标准偏差(relative standard deviations,RSDs)为0.4%~7.7%。结论该方法操作简便,灵敏、快捷,适合中草药保健食品中7种功效成分的同时测定。

Simultaneous determination of seven functional components in health-care food from Chinese herbal medicine by ultrahigh performance liquid chromatography

Objective To establish a sensitive and convenient analytical method based on ultrahigh performance liquid chromatography (UPLC) for simultaneous determination of seven functional components (including tanshinone IIA, paeoniflorin, jasminoside, paeonol, rutin, icariin and magnoflorine) in health-care food from Chinese herbal medicine. Methods The analytes were extracted by ultrasound with 60% methanol and separated by a C18 column. The mobile phase was methanol-0.1% formic acid aqueous solution with gradient elution. Uv-wavelength detection was performed, and external standard method was used for the quantitative analysis. Results Tanshinone IIA, paeoniflorin, jasminoside, rutin and magnoflorine showed good linearity when the concentration ranged from 0.50 mg/L-50.0 mg/L, while paeonol and icariin displayed good linearity over the concentration range of 0.10 mg/L-25.0 mg/L, with the correlation coefficients r≥0.9992. The method detection limits for the seven compounds were 0.1 mg/kg-1 mg/kg. The recoveries ranged from 88.9% to 105.0%. The relative standard deviations (RSDs) were in the range of 0.4%-7.7%. Conclusion The proposed method is simple, sensitive and rapid, and it is suitable for simultaneous determination of the seven functional components in health-care food from Chinese herbal medicine.