miR-1471对胶质瘤细胞增殖、侵袭和迁移能力的影响及其机制探讨

目的:探讨miR-1471对胶质瘤细胞增殖、侵袭和迁移能力的影响及其机制。方法:体外培养人胶质瘤细胞U251，将miR-1471模拟物或阴性对照分别转染至细胞中，记为miR-1471组和阴性对照（NC组），同时设置对照组。qRT-PCR检测转染效果。CCK8检测各组细胞增殖能力。Transwell实验检测各组细胞侵袭能力。划痕实验检测各组细胞迁移能力。qRT-PCR和Western blot检测转移黏附基因（metadherin, MTDH）及Wnt/β-catenin信号通路相关基因和蛋白的表达。结果:与对照组和NC组比较，miR-1471组细胞增殖活性均显著降低（P＜0.05），侵袭细胞数均显著减少（P＜0.05），划痕间距明显较大，细胞中MTDH、Wnt1和β-catenin mRNA和蛋白表达水平均显著降低（P＜0.05）。结论:miR-1471能抑制人胶质瘤细胞U251的增殖、侵袭和迁移能力，其作用机制可能与下调MTDH表达，抑制Wnt/β-catenin信号通路的激活有关。

Effect and mechanism of miR-1471 on proliferation,invasion and migration of glioma cells

Objective:To investigate the effect and mechanism of miR-1471 on proliferation,invasion and migration of glioma cells.Methods:Human glioma cells U251 were cultures in vitro.miR-1471 mimics or negative control was transfected into U251 cells,respectively,and were recorded as miR-1471 group and negative control（NC) group,respectively.Control group was set.qRT-PCR was used to detect the transfection effect.CCK8 was used to detect cell proliferation.Transwell assay was used to detect the invasive ability of cells in each group.The scratch test was used to detect the migration ability of cells in each group.qRT-PCR and Western blot were used to detect MTDH and Wnt/β-catenin signaling pathway related genes and proteins.Results:Compared with control group and NC group,the proliferation activity was significantly decreased（P＜0.05),the number of invasive cells was significantly decreased（P＜0.05),the scratch spacing increased significantly,mRNA and protein expression levels of MTDH,Wnt1 and β-catenin was significantly decreased（P＜0.05) in miR-1471 group.Conclusion:miR-1471 can inhibit the proliferation,invasion and migration of human glioma cells U251,and its mechanism may be related to down-regulation of MTDH expression and inhibition of activation of Wnt/β-catenin signaling pathway.