基于CD19/IgM亚群分析套细胞淋巴瘤骨髓中T细胞的构比异常

目的:研究T细胞在初诊IV期套细胞淋巴瘤(mantle cell lymphoma, MCL)骨髓单个核细胞中的差异表达。方法:首先流式检测健康供者及MCL组中CD19^-/IgM^-,CD19^-/IgM⁺,CD19⁺/IgM^-,CD19⁺/IgM⁺亚群的比例；其次检测CD45⁺CD3⁺T细胞在两组中的总比例；最后检测T细胞在两组四个亚群中的占比。结果:四个亚群在组内有极其显著的差异(P<0.000 1),两组间无统计学差异。T细胞在MCL组占(0.46±0.13)%较健康供者组(1.10±0.06)%显著减少(P=0.025 1)。四个亚群中T细胞占比在健康供者组内有极其显著的差异(P<0.000 1),而MCL组内差异消失(P=0.329 4)。T细胞在MCL组CD19^-/IgM^-亚组中占(0.02±0.01)%较...

The expression characteristics of T cells in mantle cell lymphoma bone marrow based on CD19/IgM sub-populations

Objective:To investigate the differential expression of T cells in bone marrow mononuclear cells of newly diagnosed stage IV mantle cell lymphoma (MCL).Methods:Firstly,the proportions of CD19-/IgM-，CD19-/IgM+，CD19+/IgM-,CD19+/IgM+ sub-populations in healthy donors and MCL group were detected by flow cytometry.Secondly,the total proportion of CD45+CD3+T cells in the two groups was detected.Finally,the ratios of T cells in the four sub-populations of the two groups were detected.Results:There were significant differences among the four sub-populations in each group (P＜0.000 1),and there was no significant difference between the two groups.The percentage of T cells in MCL group (0.46±0.13)% was significantly lower than that in healthy donor group(1.10±0.06)% (P=0.025 1).There was a significant difference in the proportion of T cells among the four sub-populations in the healthy donors group (P＜0.000 1),but such difference wasn' t found in the MCL group (P=0.329 4).The percentage of T cells in the sub-populations of CD19-/IgM- in the MCL group was (0.02±0.01)%,which was lower than that of the healthy donor group (1.51±0.35)% (P＜0.000 1).The percentage of T cells in the sub-population of CD19+/IgM- in healthy donor group and MCL group was (4.28±0.14)% and (0.65±0.45)%,respectively.The ratio of T cells in the sub-population of CD19+/IgM- in MCL group was still significantly decreased (P=0.001 2).There was no statistical difference for the other two subgroups between the two groups.Conclusion:Compared with healthy donors,the total proportion of T cells in bone marrow lesions of newly diagnosed MCL with stage IV was significantly decreased and the percentages of T cells in the sub-populations of CD19-/IgM- and CD19+/IgM-were significantly decreased.It is suggested that the lack of normal number of T cells in the bone marrow lesions of MCL,and leads to weakened immune monitoring,which may promote the occurrence and development of tumors.