上调Mg2+/Mn2+依赖性蛋白磷酸酶1F表达对鼻咽癌HONE-1细胞增殖和迁移的影响

目的 探讨上调Mg²⁺/Mn²⁺依赖性蛋白磷酸酶1F（PPM1F）对鼻咽癌HONE-1细胞增殖、迁移的影响，并阐明其作用机制。 方法 鼻咽癌HONE-1细胞分为pcDNA3.1组（转染pcDNA3.1质粒）和pcDNA3.1-Flag-PPM1F组（转染pcDNA3.1-Flag-PPM1F质粒）。实时荧光定量PCR（RT-qPCR）法和Western blotting法检测2组细胞中PPM1F和E-钙黏蛋白（E-cadherin）mRNA及蛋白表达水平，CCK-8法和克隆形成实验检测2组细胞增殖活性和克隆形成率，细胞划痕实验检测2组细胞划痕愈合率，Transwell实验检测2组细胞中迁移细胞数。 结果 与pcDNA3.1组比较，pcDNA3.1-Flag-PPM1F 组细胞中PPM1F mRNA表达水平明显升高（P<0.01），且PPM1F蛋白表达量明显增加，细胞中E-cadherin mRNA和蛋白表达水平明显升高（P<0.01），细胞增殖活性、克隆形成率和划痕愈合率明显降低（P<0.05或P<0.01），迁移细胞数明显减少（P<0.05）。 结论 上调PPM1F表达能够抑制鼻咽癌HONE-1 细胞增殖和迁移，其机制可能与细胞间的黏附作用有关。

Effects of up-regulation of protein phosphatase Mg2+/Mn2+-dependent 1F on proliferation and migration of nasopharyngeal carcinoma HONE-1 cells

Objective To investigate the effects of up-regulation of protein phosphatase Mg²⁺/Mn²⁺-dependent 1F （PPM1F） on the proliferation and migration of nasopharyngeal carcinoma HONE-1 cells， and to clarify their mechanisms. Methods The nasopharyngeal carcinoma HONE-1 cells were divided into pcDNA3.1 group （transfected with pcDNA3.1 plasmid） and pcDNA3.1-Flag-PPM1F group （transfected with pcDNA3.1-Flag-PPM1F plasmid）. The expression levels of PPM1F and E-cadherin mRNA and proteins in the cells in two groups were detected by real-time fluorescence quantitative PCR（RT-qPCR） and Western blotting methods； the activities of proliferation and clone formation rates of the cells in two groups were detected by CCK-8 method and clone formation assay； the scratch healing rates of the cells in two groups were detected by cell scratch assay，and the numbers of migation cells in two groups were detected by Transwell experiment. Results Compared with pcDNA3.1 group， the expression level of PPM1F mRNA in the cells in pcDNA3.1-Flag-PPM1F group was significantly increased（P<0.01），the expression amount of PPM1F protein was significantly increased，the expression levels of E-cadherin mRNA and protein in the cells were significantly increased（P<0.01），the proliferation activity，the clone formation rate and the scratch healing rate were significantly decreased （P<0.05 or P<0.01）， and the number of migration cells was significantly reduced（P<0.05）. Conclusion Up-regulation of PPM1F expression can inhibit the proliferation and migration of nasopharyngeal carcinoma HONE-1 cells， and its mechanism may be related to intercellular adhesion.