信号转导蛋白P65与放疗诱导口腔鳞状细胞癌细胞凋亡的关系

目的 探讨不同剂量的X射线对口腔鳞状细胞癌(OSCC)细胞中信号转导蛋白P65表达的影响以及P65与放疗诱导口腔鳞状细胞癌细胞凋亡的关系.方法 人舌鳞癌Tca8113细胞复苏后,在37℃,5%CO2的孵箱中培养.共分对照组、2、4、6、8、10 Gy剂量组,待细胞长至对数生长期后,对细胞分别应用上述照射剂量一次性照射....

Relationship between P65 and radiotherapy-induced oral squamous cell carcinoma cell line apoptosis

Objective To investigate the effects of different X-ray doses on the expression of nuclear factor-κB （NF-κB） P65 in human oral squamous cell carcinoma cell（OSCC） line and the relationship between NF-κB P65 and radiation-induced OSCC cell line apoptosis. Methods The squamous cell carcinoma of Tca8113 cell was cultivated in the 37 ℃, 5%CO2 incubator after recovery. The experiment samples were divided into six groups（control group, 2, 4, 6, 8, 10 Gy）. After growing to logarithm period, Tca8113 cells were irradiated using above-mentioned X-ray doses. The immunocyteochemistry and Western blot were used to detect the expression of NF-κB P65 after irradiation in various times（1, 3, 6, 10, 24, 48 h）. The apoptosis rates under different radiotherapy dose were detected by flow cytometer and TDT-mediated dUTP-biotin nick end labeling（TUNEL）. Results Compared with the control group, cytoplasm expression of P65 under different X-ray doses had statistically significant differences（P＜0.05）. While the cytoplasm P65 protein expression at different time were compared each other, the 3 h group demonstrated significant difference（P＜ 0.05）. Apoptosis rates in various groups, compared with control group, had statistically significant differences（P＜0.05）. While the groups at different time points were compared each other, the apoptosis rates of 3 h group had significant differences（P＜0.05）. Conclusion X-ray can activate the NF-κB P65 in oral squmaous cell carcinoma cell lines. The correlation between expressional quantity of P65 and radiotherapy induced apoptosis of oral squamous cell carcinoma cell lines possesses positive correlation. The activated and intranuclear P65 may have radiotherapy resistant effect.