陶瓷化骨对成人骨髓基质干细胞分化的影响

目的:观察陶瓷化骨时成人骨髓基质干细胞生长和分化的影响.方法:体外分离培养成人骨髓基质干细胞,将第二代细胞以陶瓷化骨为载体进行培养,利用倒置显微镜、碱性磷酸酶细胞化学染色、碱性磷酸酶活性检测等条件,了解陶瓷化骨对成人骨髓基质干细胞生物学行为的影响.结果:体外连续培养6d,倒置显微镜下可见成人骨髓基质干细胞与陶瓷化骨相容性好,材料周边细胞生长密集,并有向陶瓷化骨趋化迁移现象,3周后形成矿化结节.碱性磷酸酶染色呈阳性、碱性磷酸酶活性检测有明显的细胞分泌量.结论:体外培养条件下,陶瓷化骨可诱导成人骨髓基质干细胞向成骨和骨质细胞分化.

The effect of ceramic bovine bone on growth and differentiation of adult human bone marrow stromal cells

Objective:To observe the effect of ceramic bovine bone(CBB) on growth and differentiation of adult human bone marrow stromal cells(hBMSCs) in vitro.Methods:The hBMSCs were cultured in vitro,the second generation of cells were cultured with CBB as the carrier,inverted microscope,alkaline phosphatase(AKP) staining,ALP activity detection conditions were used to investigate effect of CBB on hBMSCs biological behavior.Results:After continuous cultivation in vitro for 6 Days,hBMSCs and CBB showed good compatibility under inverted microscope,the cells were densely distributed around the material and showed CBB chemotactic migration,the formation of mineralized nodules.AKP staining showed positive and significant secretion was detected.Conclusion:CBB could induced hBMSCs to differentiated into osteoblasts and bone cells in vitro conditions.