右美托咪啶预处理对大鼠肾缺血再灌注后肝肾抗氧化能力的影响

目的探究右美托咪啶（dexmedetomidine，DEX）预处理对大鼠肾缺血再灌注损伤后。肾及肝抗氧化能力的影响。方法90只雄性SD大鼠随机分为3组（n=30），右美托咪啶预处理组（DEX组），缺血再灌注组（IRI组），假手术组（Sham组）。DEX组于建模前30min腹腔注射DEX（100μg／ks），其余两组腹腔注射等量0．9％氯化钠注射液，建立肾缺血再灌注模型，再灌注后2h、8h、24h肝肾组织匀浆检测超氧化物歧化酶（serum levels of superoxide dismutase，SOD）、一氧化氮（nitric oxide，NO）、乳酸（lactate dehydrogenases，LD）、还原型谷胱甘肽（reduced glutathione，GSH）及总抗氧化能力（total antioxidant capacity，T—AOC）。结果与IRI组相比，DEX组肾匀浆SOD升高，总NO降低，T—AOC升高（P〈0．05），LD再灌注8h后降低明显（P〈0．05）；肝SOD升高，总NO升高，T—AOC升高（P〈0．05），LD再灌注8h、24h降低显著（P〈0．05）。结论右美托咪啶预处理可提高大鼠肾缺血再灌注后肾及肝的抗氧化能力，减少乳酸堆积。

Effect of dexmedetomidine pretreatment on antioxidant ability of kidney and liver in rats following renal ischemia/reperfusion injury

Objective To study the effect of dexmedetomidine （DEX） pretreatment on antioxidant ability of kidney and liver in rats following renal ischemia/reperfusion （I/R） injury. Methods Ninety male SD rats were randomly divided into DEX pretreatment group, UR injury group, and sham operation group （30 in each group）. DEX （100 μ g/kg） Was injecteel into the abdominal cavity of rats in DEX pretreatment group 30 min before the model was established. A renal I/R injury model was established for I/R injury group and sham operation group, respectively, by injecting 0.9% sodium chloride （100 μg/kg）. Serum levels of superoxide dismutase （SOD）, nitric oxide （NO） and lactate dehydrogenases （LD）, reduced glutathione （GSH） and total antioxidant capacity （T- AOC） in liver and renal tissues were measured 2, 8 and 24 h, respectively, after reperfusion. Results The serum SOD level and T-AOC were higher and the serum NO level was lower in DEX pretreatment group than in UR injury group （P 〈 0.05）. The serum LA level was significantly lower while the serum SOD and NO level and T-AOC were significantly higher in DEX pretreatment group than in I/ R injury group 8 h after reperfusion （P 〈 0.05） and the serum LA level was significantly lower in DEX pretreatment group than in I/ R injury group 8 and 24 h after reperfusion （P 〈 0.05）. Conclusion DEX pretreatment can increase the antioxidant ability of kidney and liver in rats following renal I/R injury, thus reducing LA accumulation.