荧光定量PCR方法检测男性三种标本的分析

目的:分析荧光定量PCR方法检测1256例前列腺液、尿道分泌物、精液标本解脲支原体(UU)和(或)沙眼衣原体(CT),了解三种标本感染UU、CT的情况。方法:对2007年1256例怀疑感染UU或CT的患者和不孕不育症患者标本检测。结果:149例前列腺标本中UU阳性率为42.3%(63/149)、136例前列腺标本CT阳性率为19.9%(27/136);361例尿道分泌物UU阳性率为21.1%(76/361)、346例尿道分泌物CT阳性率为10.4%(36/346);141例精液UU阳性率为11.3%(16/141)、123例精液CT阳性率为7.3%(9/123)。结论:三种标本的UU、CT阳性率相差显著,P<0.005。前列腺标本检测的阳性率明显高于尿道分泌物标本,精液检测的阳性率最低。不同类型的标本检测的UU、CT的结果有很大的差异,送检的标本最好是前列腺液。

Analysis of Detecting Results by FQ-PCR in Ureaplasma Urealyticum and Chlamydin Trachomatis From 3 Types of Samplse of Male

Objective To analyze 1 256 samples of Prostate liquid ,the secretion of the Urinous tract, and Semen which are tested by FQ -PCR,To investigate which infected UU or CT from 3 types of samples. Methods Test 1 256 samples from suspect UU or CT sufferers and infertile patients during 2007. Results The positive rate of UU of 149 samples of Prostate liquid is 42.3%（63/149）, and The positive rate of CT of 136 samples of Prostate liquid s is 19.9%（27/136）;The positive rate ofUU of 361samples of the secretion of the Urinous tract is 21.1% （76/361）,and The positive rate of CT of 346 samples of the secretion of the Urinous tract is 10.4%（36/346）;The positive rate of UU of 141 samples of Semen is 11.3% （16/141）,and The positive rate of CT of 123 samples of Semen is 7.3%（9/123）. Conclusion There are significant differences in positive rate of UU and CT of 3 types of samples P〈0.005.The positive rate of specimens of Postate liquid is higher than that of the secretion of the Urinous tract ,the positive rate of Semen test is the lowest..The results from the UU and CT test of different types of specimens are different obviously .The most suitable specimen of test is Prostate liquid.