| 吴茱萸收缩血管作用的体内体外研究(英文) |
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| 引用本文： | 王秀坤,王玉刚,詹宏磊,柴玉爽,胡珺,邢东明,游学甫,雷帆,杜力军. 吴茱萸收缩血管作用的体内体外研究(英文)[J]. 中国天然药物, 2011, 0(1): 65-73 |
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| 作者姓名： | 王秀坤 王玉刚 詹宏磊 柴玉爽 胡珺 邢东明 游学甫 雷帆 杜力军 |
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| 作者单位： | 清华大学生命科学学院医学院药物药理研究室;中国医学科学院中国协和医科大学医药生物技术研究所药理室; |
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| 基金项目： | 国家十一五科技支撑计划项目(Nos. 90713043,30801523,30973896); 中国博士后科学基金(No.20080440418); 国家十一五“重大新药创制”科技重大专项(Nos. 2009ZX09103-301,2009ZX09502)~~ |
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| 摘 要： | 目的:对吴茱萸的收缩血管作用及其机制进行了研究。方法:吴茱萸提取物经透析袋(100D)处理后,对吴茱萸透析液(ED,>100D)进行了体内和体外实验研究。结果:ED能够明显增加小鼠脑血流,并能够诱导大鼠胸主动脉环的收缩,且均具有一定的剂量效应。在苯肾上腺素收缩血管的基础上ED能够引起进一步的收缩反应。250mg·L-1 ED的血管收缩活性,其诱导血管收缩的程度是50mmol·L-1 KCl最大收缩的113%,是10μmol·L-1苯肾上腺素的78%,为20mmol·L-1咖啡因的183%。细胞表面α受体的拮抗剂酚妥拉明,能够明显抑制吴茱萸收缩血管活性,而β,M和血管紧张素Ⅱ受体拮抗剂普萘洛尔、阿托品和氯沙坦均未对ED的收缩血管活性带来影响。去除细胞外的钙离子或者使用L-型钙离子通道拮抗剂尼卡地平可以显著地抑制ED的缩血管作用,而钙离子通道开放剂BAY-K8644和钾离子通道开放剂吡那地尔对ED收缩血管作用也有一定影响。利用选择性受体抑制剂对ED可能的信号通路研究显示,使用肌球蛋白激酶(MLCK)抑制剂、RhoA抑制剂和三磷酸肌醇(IP3)受体抑制剂均可以抑制ED的活性,而蛋白激酶C(PKC)抑制剂和胞外信号调...
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| 关 键 词： | 吴茱萸 透析 血管收缩 |
Comprehensive Study of Evodia rutaecarpa-induced Contraction on Blood Vascular in Vivo and in Vitro |
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| WANG Xiu-Kun ,WANG Yu-Gang ,ZHAN Hong-Lei ,CHAI Yu-Shuang ,HU Jun ,XING Dong-Ming ,YOU Xue-Fu ,LEI Fan ,DU Li-Jun Protein Science Laboratory of the Ministry of Education,Laboratory of Pharmaceutical Sciences,School of Life Sciences , School of Medicine,Tsinghua University,Beijing ,China. Comprehensive Study of Evodia rutaecarpa-induced Contraction on Blood Vascular in Vivo and in Vitro[J]. Chinese JOurnal of Natural Medicines, 2011, 0(1): 65-73 |
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| Authors: | WANG Xiu-Kun WANG Yu-Gang ZHAN Hong-Lei CHAI Yu-Shuang HU Jun XING Dong-Ming YOU Xue-Fu LEI Fan DU Li-Jun Protein Science Laboratory of the Ministry of Education Laboratory of Pharmaceutical Sciences School of Life Sciences School of Medicine Tsinghua University Beijing China |
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| Affiliation: | WANG Xiu-Kun 1,2,WANG Yu-Gang 1,ZHAN Hong-Lei 1,CHAI Yu-Shuang 1,HU Jun 1,XING Dong-Ming 1,YOU Xue-Fu 2,LEI Fan 1,DU Li-Jun 1* 1 Protein Science Laboratory of the Ministry of Education,Laboratory of Pharmaceutical Sciences,School of Life Sciences and School of Medicine,Tsinghua University,Beijing 100084,China,2 Institute of Medicinal Biotechnology,Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100050 |
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| Abstract: | AIM:Evodiae Fructus (fruit of Evodia rutaecarpa),a blood vessel activator,has been used,with headache,migraine,shock etc.,for a long history in Chinese medical practice.However,previous studies supported the vessel relaxation and the potential action on cardiac and cerebral blood circulation of vessel contraction.The present study was designed to explore it effects on contraction and the relative mechanism.METHODS:Evodiae Fructus was extracted by dialysis tubing (in diameter of 100 Dalton cutoff) and the ef... |
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| Keywords: | Evodia rutaecarpa Dialysis Blood vessel contraction |
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