茵陈蒿汤及其变方对ConA诱导慢性免疫性肝损伤小鼠IP-10、CXCR3的影响

目的:观察茵陈蒿汤及其变方对刀豆蛋白(concanavalin A,ConA)诱导小鼠慢性免疫性肝损伤的保护作用.方法:选用Balb/c小鼠70只,雌雄各半,随机分为正常对照组、模型组、茵陈蒿汤组、茵陈蒿汤加丹参组、茵陈蒿汤加黄芪组、荣肝合剂组和荣肝小方组.正常组小鼠尾静脉注射PBS溶液0.3 mL,其他各组按ConA 6 g/g体质量尾静脉注射造模,1次/wk,连续8 wk,制备慢性肝损伤模型.造模成功后,模型组和正常组以等体积蒸馏水灌胃.各治疗组灌胃给药,每日1次,连续4 wk,末次给药后24 h,检测血清中IP-10、CXCR-3和TNF-活性;肝组织光镜下观察其病理变化.结果:与模型组比较,各用药组(茵陈蒿组、茵陈蒿汤加丹参组、茵陈蒿汤加黄芪组、荣肝合剂组和小方组)血清IP-10、CXCR-3、TNF-的水平(ng/mL)均降低(IP-10:44.56±0.30、42.18±0.54、32.18±0.37、36.46±0.47、35.98±0.65 vs 52.73±0.46;CXCR-3:64.86±0.63、37.88±0.63、40.18±0.57、38.58±0.62、36.94±0.26 vs 64.86±0.63;T N T-:43.45±0.65、32.78±0.27、42.18±0.37、28.69±0.85、24.46±0.57 vs 93.32±0.81,均P<0.05),各组间无统计学差异(P>0.05);与模型组比较,中药组肝细胞坏死减轻、变性减少、炎细胞浸润和肝纤维化减轻(均P<0.05);茵陈蒿汤加丹参组、荣肝合剂组和小方组肝组织纤维增生程度较小(均P<0.05).结论:清利湿热,健脾活血的荣肝合剂及荣肝小方组对ConA所致小鼠肝损伤具有较好的综合保护作用.

Effect of modified decoction of Yinchenhaotang on IP-10 and CXCR3 expression in concanavalin A-induced liver injury in mice

AIM: To observe the effect of modified decoction of Yinchenhaotang on concanavalin A(ConA) induced chronic hepatic injury in mice.METHODS: Seventy Balb/c mice were randomly divided into 7 groups: normal group,model group,Yinchenhaotang group,Yinchenhaotang plus salvia miltiorrhiza group,Ynchenhaotang plus milkvetch root group,Rongganheji group,and Rongganxiaofang group.Mice in the normal group were injected with 0.3 mL of PBS solution via the tail vein,while mice in other groups were injected with ConA at a dose of 6 g/g of body weight once a week for 8 wk.Mice in each groups were administrated with each treatment reagent for 4 wk,and the same volume of normal saline was given to the normal and model groups.Twenty-four hours after the last administration,blood samples were taken to determine the levels of IP-10,CXCR3 and TNF-.The degree of inflammation and fibrosis was observed under a light microscope.RESULTS: Serum levels of IP-10,CXCR3 and TNF-(ng/mL) were reduced in all the treatment groups(Yinchenhaotang group,Yinchenhaotang plus salvia miltiorrhiza group,Ynchenhaotang plus milkvetch root group,Rongganheji group,and Rongganxiaofang group) compared to the model group(IP-10: 44.56 ± 0.30,42.18 ± 0.54,32.18 ± 0.37,36.46 ± 0.47,35.98 ± 0.65 vs 52.73 ± 0.46;CXCR-3: 64.86 ± 0.63,37.88 ± 0.63,40.18 ± 0.57,38.58 ± 0.62,36.94 ± 0.26 vs 64.86 ± 0.63;TNT-: 43.45 ± 0.65,32.78 ± 0.27,42.18 ± 0.37,28.69 ± 0.85,24.46 ± 0.57 vs 93.32 ± 0.81,all P<0.05).Compared to the model group,liver fibrosis and cell necrosis were significantly improved in Rongganheji and Yinchenhaotang groups(all P < 0.05).CONCLUSION: Rongganheji has a good protective effect against concanavalin A-induced liver injury in mice.