肝素治疗烧伤的蛋白质组学研究

[目的]利用蛋白质组学的方法,寻找肝素处理烧伤后差异表达的蛋白质,进一步探索肝素治疗烫伤过程的相关蛋白质,以期阐明肝素治疗烫伤的机制。[方法]制作小鼠Ⅱ度烫伤模型,使用肝素外敷治疗,并在第3天从肝素组（烫伤后用肝素治疗）、烫伤组（烫伤后自然愈合）和对照组（未进行烫伤处理）中各取5只小鼠处死,提取烫伤及其周围皮肤组织的蛋白质,采用双向凝胶电泳（2-DE）技术分离各组的蛋白质,用考马斯亮蓝方法染色。通过凝胶成像系统获得双向电泳凝胶图谱后,用PD Quest图像分析软件比较分析,从而确定差异表达的蛋白质。选取差异最明显的蛋白质点,经胰蛋白酶水解后,通过基质辅助激光解吸附飞行时间串联质谱（MALD I-TOF-MS/MS）对其进行肽质谱分析并与数据库比对,鉴定待测蛋白质。[结果]烫伤组共检测出75个蛋白质点,肝素组共检测出95个蛋白质点,其中肝素处理后上调2倍以上的蛋白质点有10个,下调2倍以上的点有4个。质谱分析初步鉴定表达差异最大的蛋白质点为载脂蛋白A-I前体。[结论]烫伤创面经肝素处理后表现出不同的蛋白质表达谱。

Proteomic study on heparin treated burns

[Objective] To identify the differentially expressed proteins from burns after treatment with heparin for elucidation of the mechanism of heparin treatment on burns.[Methods] Second-degree burned mice were divided into three groups randomly: Heparin treated group(3000 U/mL heparin after burning),burned group(the wound without treatment) and control group(no burns).Three day after heparin treatment,the total proteins from the three groups were extracted and separated by two-dimensional gel electrophoresis(2-...