Bcl-2 siRNA对淋巴瘤细胞系CA46凋亡的影响

本研究探讨bcl-2 siRNA对淋巴瘤细胞系CA46凋亡及bcl-2基因表达的影响。设计并合成1条siRNA，用阳离子脂质体法转染CA46细胞。转染后48小时收集CAd6细胞，用流式细胞仪检测细胞凋亡及凋亡过程中线粒体跨膜电位改变，用RT—PCR方法和流式细胞仪分别检测CA46细胞bcl-2 mRNA和BCL-2蛋白的表达。结果表明：bcl-2 siRNA转染CAd6细胞48小时后，CA46细胞出现凋亡且线粒体跨膜电位发生变化；bcl-2 mRNA和BCL-2蛋白表达均显著降低。结论：bcl-2 siRNA可特异性抑制淋巴瘤细胞CA46 bcl-2基因的表达，改变CA46细胞线粒体跨膜电位诱导其凋亡。

Effect of bcl-2 siRNA on Apoptosis of Burkitt's Lymphoma Cell Line CA46

The aim of this study was to investigate the effect of bcl-2 siRNA on bcl-2 gene expression and apoptosis of lyrnphoma cell line CA46. A siRNA was designed and synthesized. Then siRNA was transfected into CA46 cells by cationic liposome. At 48 hours after transfection, apoptosis and mitochondria transmembrane potential of CA46 cells were detected by flow cytometry, the expression of bcl-2 mRNA and BCL-2 protein in CA46 cells were detected by RTPCR and flow cytometry respectively. The results showed that at 48 hours after transfection, apoptosis of CA46 cells occurred, mitochondria transmembrane potential changed. The expression of bcl-2 mRNA and BCL-2 protein in CA46 cells decreased significantly. In conclusion, bcl-2 siRNA depresses the expression of bcl-2 gene in CA46 cells specifically, then changes the mitochondria transmembrane potential, resulting in apoptosis of CA46 cells.