骨髓间充质干细胞移植后的肝功能重建

背景：细胞移植是治疗肝功能衰竭的一种有效方法，而理想的细胞源和最佳的移植途径一直是各实验室不断研究的目标。 目的：观察骨髓间充质干细胞移植对急性肝衰竭大鼠肝功能重建的影响和最佳移植途径。 方法：以D-氨基半乳糖胺作为肝脏毒剂，构建雌性SD大鼠急性肝衰竭模型。以雄性SD大鼠骨髓间充质干细胞为细胞源，通过腹股沟静脉和脾内移植两种途径植入肝衰竭大鼠，动态检测多项肝功能血生化指标。并以sry基因为分子标志，采用PCR技术检测骨髓间充质干细胞在肝脏内的存活状态。 结果与结论：骨髓间充质干细胞经腹股沟静脉和脾内移植均可降低血清谷丙转氨酶、谷草转氨酶、总胆红素水平，提高白蛋白合成能力，改善肝功能，降低动物死亡率。经腹股沟静脉移植组死亡率低于经脾内移植组。移植后7~30 d在受体大鼠肝脏内均能检测到sry基因阳性细胞的存在，而相应对照组未能检测到sry基因阳性细胞。结果表明①骨髓间充质干细胞移植可改善急性肝衰竭大鼠的肝功能。②骨髓间充质干细胞可在受体肝脏内存活。③经腹股沟静脉移植操作简便易行，死亡率较低，是较好的细胞移植途径

Reconstruction of hepatic functions after bone marrow mesenchymal stem cells transplantation

BACKGROUND: Cell transplantation is an effective method for the treatment of hepatic function failure. However, ideal cell source and the best way to transplant has been the target of the ongoing research in laboratories. OBJECTIVE: To observe the effect and optimal transplantation way of bone marrow mesenchymal stem cells (BMSCs) transplantation on hepatic function reconstruction with acute hepatic failure in rats. METHODS: D-galactosamine (D-gal) was served as hepatic toxic agent, female SD rats with acute hepatic failure model was constructed. Male rats BMSCs as cell source was implanted hepatic failure rats through groin vein and intrasplenic transplantation. Many hepatic function blood biochemical indices were detected dynamically. Sry gene was served as molecule marker, the survival status of BMSCs in hepatic was detected by PCR technique. RESULTS AND CONCLUSION: BMSCs through groin vein and intrasplenic transplantation can lower the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBIL), improve the ability of albumin (ALB) synthesis, ameliorate hepatic function, and reduce the death rate of acute hepatic failure rats. The death rate in the group of groin venous transplantation was lower than that in the intraspleen transplantation group. Sry gene positive cell was detected in hepatic of receptor rats at 7-30 days after transplantation; however, sry gene positive cell was not detected in hepatic of receptor rats in corresponding control group. The results showed that BMSCs can ameliorate hepatic function of acute hepatic failure rats. BMSCs can survive in the hepatic of receptor rats. The operation of groin venous transplantation is simple and convenient; the death rate is lower. So groin venous transplantation is a better way to treat acute hepatic failure rats.