脑内和肌内接种小鼠胶质母细胞瘤株后神经末梢的观察

本实验应用Nonidez及Glees二种镀银法,对诱发的小鼠胶质母细胞瘤株(G 422)进行了观察。脑内及肌内接种胶质母细胞瘤后,在肿瘤边缘可见大小不等的神经束,伴随或不伴随血管伸入瘤内。有的在血管周围间隙形成血管周围神经丛。这些神经纤维与肿瘤周围宿主的脑组织、皮下、毛囊和肌肉间隙的神经纤维相联系。因此我们推测肿瘤内的神经是由肿瘤周围宿主的器官组织的神经延伸来的。肿瘤边缘的神经纤维的数量多于核心区,走行于肿瘤的间质或实质,沿途不断分支,终末分布到肿瘤细胞的表面。我们观察到球形、游离分叉状、梭形、环形、树枝状、杵状及丛刷状等类型的神经末梢。以上观察表明,恶性肿瘤——小鼠胶质母细胞瘤是受神经支配的。

OBSERVATION ON NERVE TERMINALS IN THE GLIOBLASTOMA OF MOUSE INOCULATED INTO BRAIN AND MUSCLE

An observation of nerve terminals in the induced glioblastoma of mouse was made by Nonidez and Glees impregnation method.In the marginal zones of the glioblastoma innoculated in the brain or muscle,nerve fascicles of various sizes with or without the blood vessels could be seen. In some case the nerve fibers formed a perivascular plexus through the perivascular space and some nerve fibers terminated on vascular walls.The nerve fibers within the tumor seemed to be related to those nerve fibers distributed in the brain tissue near the tumor or in the subcutaneous tissues, hair follicules of muscles around the tumor, hence, we suggest that the nerves whthin the tumor were growing in from the surrounding normal tissues.The amount of the nerve fibers was more abundant in the periphery than in the center of the tumor, the nerve fibers ran through the stroma or parenchyma of the tumor and gave out many branches to the tumor cells, we have also observed various types of nerve terminals for example: bulbar, isolated bifurcated, fusiform,circular, branched, brush-formed and club-formed endings.According to the findings mentioned above, it is concluded that the induced glioblastoma of mouse is innervated by nerve endings.