遗传学诊断在胚胎移植过程中的临床价值

目的评估遗传学诊断在胚胎移植过程中的临床价值。方法采用广州军区广州总医院生殖中心2009年2月—2012年1月期间的废弃胚胎,由D2期培养到D3期,采用荧光原位杂交技术检测胚胎固定后细胞核内的X、Y、13、18和21号染色体异常情况,采用高形态学评分的D3作为对照。结果 A组D2期胚胎为多核(卵裂球核≥2个)共22枚,染色体数目异常胚胎16枚,异常率为71.98%。与对照组比较有显著性差异(P<0.05)。B组胚胎D2期多核(卵裂球核=2个)21枚,胚胎染色体变异数目15枚,异常率为72.23%,与对照组比较有显著性差异(P<0.05),A组与B组间比较没有统计学差异。结论 D2期多核胚胎发展到D3期异常率高,不建议使用此类胚胎进行临床移植。

Research of genetic diagnosis application in embryo transplantation

Objective Access the clinical value of Preimplantation genetic diagnosis（PGD） in the process of Embryo transplantation.Methods Select the abandoned D2 embryo from reproductive center of city hospital between the period of Feb.2009 to Jan.2012.Detect the abnormal of X、Y、13、18 and 21 chromosome through FISH,take the good morphology D3 group as control.Results There were 22 embryos in group A whose D2 embryo is Multinucleated（Blastomere nucleus≥2）,16 embryos were chromosome abnormal and the abnormal rate was 71.98%.,Significant differences were exist compared with the controlled group（p0.05）.21 D2 Multinucleated embryos in group B（Blastomere nucleus=2）,15 embryos were chromosome abnormal and the abnormal rate was 72.23%,there were Significant differences compared with the controlled group（P0.05）.There were no statistical deference between group A and group B（P0.05）.Conclusion The development of D2 Multinucleated embryos to D3 derives high abnormal rates,we don＇t suggest choosing those embryos to conduct Embryo transplantation.