瘢痕组织细胞内肌动蛋白的实验研究

目的 探讨增生性瘢痕疙瘩成纤维细胞中肌动蛋白（ａｃｔｉｎ）与瘢痕挛缩的关系。方法取增生性瘢痕１０例,瘢痕疙瘩５例,对成纤维细胞进行培养,并抽提细胞内蛋白成份后用ＳＤＳ－ＰＡＧＥ电泳,然后通过凝胶扫描仪测定两种组织来源的成纤维细胞内的总肌动蛋白、纤维状肌动蛋白（Ｆ肌动蛋白）、球形肌动蛋白（Ｇ肌动蛋白）的量,并计算Ｆ／Ｇ肌动蛋白的比值。结果 增生性瘢痕细胞每１０＾４个细胞内含总肌动蛋白,Ｆ肌动蛋白、Ｇ

THE EXPERIMENTAL STUDY OF INTRACELLULAR ACTIN IN THE SCAR FIBROBLASTS

Objective To study the relationship between intracellular actin and scar contracture. Methods Fibroblasts from 10 cases of hypertrophic scar and 5 cases of keloid were cultured in vitro . Total actin, filamentous actin(F actin), globular actin (G actin) and the ratio of F to G actin(F/G) were measured by densitometry after differential extraction and seperation by polyacrylamide gel electrophoresis in the presence of sodium sulfate. Results Total actin, F actin, G actin and F/G in hypertrophic scar fibroblasts were 2.38 ng/10 4 cells, 0.98 ng/10 4 cells, 1.42 ng/10 4 cells and 0.68 respectively, while in keloid fibroblasts were 1.68 ng/10 4 cells. 0.46 ng/10 4 cells, 1.26 ng/10 4 cells, and 0.36 respectively. There was significant differences between two tissues fibroblasts in the items of total actin, F actin, G actin, and F/G ( P<0.01) , while no significant difference in G actin( P>0.05) . Conclusion Total intracellular actin, F actin, and F/G may play an important role in the scar contracture. The hypertrophic scar and keloid can be distinguished by the contents of total intracellular actin, F actin and F/G.